Immobilization of α-amylase in ethylcellulose electrospun fibers using emulsion-electrospinning method

• Published in: Carbohydrate polymers 2022-02, Vol.278, p.118919-118919, Article 118919
• Main Authors: Hosseini, Asad, Ramezani, Soghra, Tabibiazar, Mahnaz, Mohammadi, Maryam, Golchinfar, Zahra, Mahmoudzadeh, Maryam, Jahanban-Esfahlan, Ali
• Format: Article
• Language: English
• Subjects: alpha-Amylases - metabolism; Cellulose - analogs & derivatives; Cellulose - metabolism; Electrospun fibers; Emulsions; Enzyme immobilization; Enzymes, Immobilized - metabolism; Hydrogen-Ion Concentration; Polyethylene Glycols - metabolism; Temperature; α-Amylase
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2021.118919

α-Amylase encapsulated in water in oil (W/O) emulsion was prepared using poly ethylene glycol (PEG 10000) (2%w/v) as water phase and ethylcellulose (EC) in ethyl acetate as oil phase at the ratio of 10:90 v/v. Next, the electrospun fibers were prepared by mixing enzyme loaded emulsion with EC solution (20%w/v) in acetic acid/ethyl acetate (20:80 v/v) at the 2:1 ratio. The emulsion showed good physical stability. The immobilized enzyme showed high activity across a board range of pHs and temperatures. The storage stability of the immobilized enzyme was 2 fold of free enzyme activity after 45 days. The residual activity of immobilized α–amylase onto of fibers after 10 and 15 repeated cycles, was approximately 100% and 50%, respectively. The results of this study indicated that the α–amylase loaded EC fibers have acceptable activity against harsh conditions and excellent reusability. [Display omitted]