CD44-positive cancer stem cells from oral squamous cell carcinoma exhibit reduced proliferation and stemness gene expression upon adipogenic induction

We proposed to assess adipogenic differentiation and its effect on the proliferation and stemness markers in CD44 + OSCC CSCs. D44 + CSCs were sorted by magnetic sorting from the single-cell suspension of the OSCC tumor. Adipogenic differentiation was induced by an adipogenic induction medium. Lipid...

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Bibliographic Details
Published in:Medical oncology (Northwood, London, England) London, England), 2022-02, Vol.39 (2), p.23-23, Article 23
Main Authors: Patil, Shankargouda, Al-Brakati, Ashraf, Abidi, Nazim H., Almasri, Mazen A., Almeslet, Asma saleh, Patil, Vikrant R., Raj, A. Thirumal, Bhandi, Shilpa
Format: Article
Language:English
Subjects:
Adipocytes
Adipogenesis
Biomarkers, Tumor - genetics
Cancer
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - pathology
Cell Differentiation
Cell Line, Tumor
Cell Proliferation
Down-Regulation
Flow Cytometry
Gene expression
Gene Expression Regulation, Neoplastic
Hematology
Humans
Hyaluronan Receptors
Internal Medicine
Ki-67 Antigen - analysis
Medicine
Medicine & Public Health
Mouth Neoplasms - genetics
Mouth Neoplasms - pathology
Neoplastic Stem Cells - pathology
Oncology
Oral cancer
Original Paper
Pathology
Squamous cell carcinoma
Stem cells
Tumors
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Summary:We proposed to assess adipogenic differentiation and its effect on the proliferation and stemness markers in CD44 + OSCC CSCs. D44 + CSCs were sorted by magnetic sorting from the single-cell suspension of the OSCC tumor. Adipogenic differentiation was induced by an adipogenic induction medium. Lipid droplet formation was confirmed by oil red O staining. The expression of the cell surface marker was analyzed by flow cytometry. Real-time qPCR was performed to examine the gene expression activity. CD44 + OSCC CSCs can differentiate into adipocytes and adipogenesis in these cells decrease their proliferation and stemness gene expression. Adipogenic induction can make the cancer stem cells from OSCC tumors lose their stemness potential. Oral cancer, especially OSCC, is a huge burden worldwide. Similar to other stem cells, cancer stem cells can differentiate into other lineage cells. Our study shows that the proliferation and stemness gene expression in the CSCs from OSCC tumors can be thwarted by inducing them to differentiate into adipocytes, which could be advantageous to find out new clinical approaches in the treatment of cancers, like OSCC. Graphical abstract
ISSN:1357-0560
1559-131X
DOI:10.1007/s12032-021-01617-4