Lederbergia citri sp. nov., and Lederbergia citrisecundus sp. nov., isolated from citrus rhizosphere

• Published in: Current microbiology 2022-02, Vol.79 (2), p.43-43, Article 43
• Main Authors: Liu, Guo-Hong, Narsing Rao, Manik Prabhu, Chen, Qian-Qian, Che, Jian-Mei, Shi, Huai, Liu, Bo, Li, Wen-Jun
• Format: Article
• Language: English
• Subjects: Bacterial Typing Techniques; Biomedical and Life Sciences; Biotechnology; Cardiolipin; Citrus; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Fatty Acids; Genomes; Glycerol; Hybridization; Laboratories; Lederbergia; Life Sciences; Lipids; Microbiology; New species; Nucleotides; Penicillin; Peptidoglycans; pH effects; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids; Phylogenetics; Phylogeny; Quinones; Rhizosphere; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Soil Microbiology
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-021-02741-0

Two Gram-positive, rod-shaped, motile, endospore-forming strains, FJAT-49780 T and FJAT-49732 T were isolated from a citrus rhizosphere soil sample. The optimal growth temperatures for strains FJAT-49780 T and FJAT-49732 T were 45 and 35–40 °C, respectively. The optimal growth pH for strains FJAT-49732 T and FJAT-49780 T were pH 8.0 and pH 6.0, respectively. The 16S rRNA gene sequence similarity between FJAT-49780 T and FJAT-49732 T was 98.6%. Strains FJAT-49780 T and FJAT-49732 T shared 97.9–98.4% 16S rRNA gene sequence similarities to the type strain of Lederbergia wuyishanensis . In phylogenetic trees (based on 16S rRNA gene sequence), strains FJAT-49732 T and FJAT-49780 T clade with Lederbergia members. Both strains contained meso -diaminopimelic acid in their cell-wall peptidoglycan and MK-7 was the only isoprenoid quinone detected. The major fatty acids of strains FJAT-49732 T and FJAT-49780 T were anteiso-C 15:0 and iso-C 15:0 . The polar lipids of strain FJAT-49780 T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, unidentified phospholipid and unidentified lipids while strain FJAT-49732 T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified glycolipid, unidentified aminolipid and unidentified phospholipid. The genomic DNA G+C content of strains FJAT-49780 T and FJAT-49732 T were 37.0 and 36.7%, respectively. The digital DNA–DNA hybridization and average nucleotide identity values between strains FJAT-49780 T and FJAT-49732 T and with other members of the genus Lederbergia were below the cut-off level for species delineation. Thus, based on the above results, strains FJAT-49780 T and FJAT-49732 T represent two novel species of the genus Lederbergia , for which the names Lederbergia citri sp. nov., and Lederbergia citrisecundus sp. nov., are proposed. The type strains are FJAT-49780 T (= CCTCC AB 2019242 T  = LMG 31583 T ) and FJAT-49732 T (= CCTCC AB 2019246 T  = LMG 31584 T ).