Analysis of biochemical features of ST8 α-N-acetyl-neuraminide α2,8-sialyltransferase (St8sia) 5 isoforms

Gangliosides are important components of the membrane and are involved in many biological activities. St8sia5 is an α2,8-sialyltransferase involved in ganglioside synthesis, and has three isoforms. In this study, we analyzed the features of three isoforms, St8sia5-S, -M, and -L that had not been ana...

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Published in:Glycoconjugate journal 2022-04, Vol.39 (2), p.291-302
Main Authors: Araki, Erino, Hane, Masaya, Hatanaka, Rina, Kimura, Ryota, Tsuda, Kana, Konishi, Miku, Komura, Naoko, Ando, Hiromune, Kitajima, Ken, Sato, Chihiro
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biomedical and Life Sciences
Gangliosides
Gangliosides - metabolism
Golgi apparatus
Golgi Apparatus - metabolism
Isoforms
Life Sciences
Localization
Mice
Original Article
Pathology
Protein Isoforms - genetics
Sialyltransferases - genetics
Sialyltransferases - metabolism
Tribute to Professor Sen-itiroh Hakomori
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Summary:Gangliosides are important components of the membrane and are involved in many biological activities. St8sia5 is an α2,8-sialyltransferase involved in ganglioside synthesis, and has three isoforms. In this study, we analyzed the features of three isoforms, St8sia5-S, -M, and -L that had not been analyzed, and found that only St8sia5-L was localized in the Golgi, while the majority of St8sia5-M and -S were localized in the ER. The localization of Golgi of St8sia5 depended on the stem region. In addition, the incorporation of exogenous GD3 was upregulated only in St8sia5-L expressing cells. Taken together, the localization of St8sia5 is important for the activity of the enzyme.
ISSN:0282-0080
1573-4986
DOI:10.1007/s10719-021-10034-8