Identification of polyphenols from Rosa roxburghii Tratt pomace and evaluation of in vitro and in vivo antioxidant activity

• Published in: Food chemistry 2022-05, Vol.377, p.131922-131922, Article 131922
• Main Authors: Huang, Dansi, Li, Chao, Chen, Qing, Xie, Xing, Fu, Xiong, Chen, Chun, Huang, Qiang, Huang, Zebo, Dong, Hao
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant activity; Antioxidants - pharmacology; Caenorhabditis elegans; Chromatography, High Pressure Liquid; Nematoda - drug effects; Phenolics; Plant Extracts - pharmacology; Polyphenols - pharmacology; Pomace; Rosa - chemistry; Rosa roxburghii; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2021.131922

•Free and bound phenolic fractions were extracted from Rosa roxburghii Tratt pomace.•Free and bound polyphenols were identified in RRTP-FPF and RRTP-BPF.•RRTP-BPF and RRTP-FPF could scavenge radicals in a dose-dependent manner.•RRTP-BPF and RRTP-FPF could improve SOD and CAT activity in the living C. elegans.•Our results imply that R. roxburghii pomace is a promising source of antioxidants. Rosa roxburghii Tratt pomace (RRTP) has increasingly attracted attention due to its various nutritional ingredients and health benefits. In this study, the free phenolic fraction (RRTP-FPF) and bound phenolic fraction (RRTP-BPF) were extracted from RRTP by solvent extraction method and alkaline hydrolysis method, respectively. The composition of polyphenols in RRTP-FPF and RRTP-BPF were identified by ultra-high performance liquid chromatography equipped with an electrospray ionization and quadrupole time-of-flight mass spectrometry (UHPLC-ESI-QTOF-MS/MS). In vitro antioxidant assays indicated that RRTP-FPF and RRTP-BPF could scavenge radicals in a dose-dependent manner, and RRTP-BPF exhibited better scavenging activity than RRTP-FPF. In addition, RRTP-FPF and RRTP-BPF (20 ∼ 100 μg/mL) treatment for 24 h could significantly increase the survival rate and decrease reactive oxygen species (ROS) level of paraquat-exposed nematodes through improving the activities of superoxide dismutase (SOD) and catalase (CAT). These results suggest that RRTP could be as a good and cheap source of natural antioxidants.