Removal effect of DNA contamination by hydrogen peroxide plasma compared to ethylene-oxide gas

•Hydrogen peroxide plasma treatment is useful for removing DNA contamination.•Hydrogen peroxide plasma treatment can be applied under ISO 18385.•It is suggested that part of ISO 18385 be revised. We examined the ability of hydrogen peroxide plasma (HPP) to remove DNA contamination, to evaluate wheth...

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Bibliographic Details
Published in:Legal medicine (Tokyo, Japan) Japan), 2022-02, Vol.54, p.102009-102009, Article 102009
Main Authors: Hashiyada, Masaki, Nakanishi, Hiroaki, Asogawa, Minoru, Akane, Atsushi, Saito, Kazuyuki, Miyachi, Hayato, Osawa, Motoki
Format: Article
Language:English
Subjects:
DNA
DNA Contamination
Ethylene Oxide
Ethylene-oxide gas
Ethylenes
Hydrogen Peroxide
Hydrogen peroxide plasma
ISO 18385
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Summary:•Hydrogen peroxide plasma treatment is useful for removing DNA contamination.•Hydrogen peroxide plasma treatment can be applied under ISO 18385.•It is suggested that part of ISO 18385 be revised. We examined the ability of hydrogen peroxide plasma (HPP) to remove DNA contamination, to evaluate whether it is a suitable forensic-grade treatment under ISO 18385. HPP treatment was compared to ethylene-oxide gas (EOG) treatment, which is required by ISO 18385. For the evaluation, commercial control DNA solution and cultured cells sprinkled on Petri dishes were used, and the DNA fragments (214 and 80 bp autosomal DNA fragments and 75 bp Y chromosome fragment) were quantified. HPP treatment was performed up to four times and EOG treatment was performed once. Performing HPP treatment three times was as effective as EOG treatment, with all fragments decreasing to below 1/1,000 in DNA solution. With STR and Y-STR typing, no alleles were detected for three HPP treatments of control DNA using the original amount, i.e., 1 ng. Therefore, HPP appears useful for removing DNA contamination. For cells sprinkled on Petri dishes, the DNA degradation abilities of the HPP and EOG were comparable. However, less DNA was degraded with the HPP and EOG and neither met the ISO criteria. Although the current version of ISO 18385 recommends an evaluation method using cultured cells sprinkled on Petri dishes, it needs to be revised. These findings should be considered when revising ISO 18385.
ISSN:1344-6223
1873-4162
DOI:10.1016/j.legalmed.2021.102009