Adsorption, separation, and purification of cyclosporine A using reversed-phase liquid chromatography

High performance liquid chromatography (HPLC) is widely used in the separation and analysis of cyclosporine A (CsA). Analyzing the chromatographic behavior of CsA is key to the purification of CsA by preparative HPLC. In this study, the retention behavior of CsA on the C18 column using mobile phases...

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Bibliographic Details
Published in:Sepu 2022, Vol.40 (1), p.66-73
Main Authors: Li, Zhidong, Fu, Qing, Dai, Zhuoshun, Jin, Yu, Liang, Xinmiao
Format: Article
Language:Chinese
Subjects:
Adsorption
Chromatography, High Pressure Liquid
Chromatography, Reverse-Phase
Cyclosporine
Solvents
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Summary:High performance liquid chromatography (HPLC) is widely used in the separation and analysis of cyclosporine A (CsA). Analyzing the chromatographic behavior of CsA is key to the purification of CsA by preparative HPLC. In this study, the retention behavior of CsA on the C18 column using mobile phases of methanol-water and acetonitrile-water was compared. The retention time of CsA was sensitive to the change in the ratio of the organic solvent. When 84%-88% methanol or 75%-85% acetonitrile was used, the retention factor ( ) was in the range of 3-7. The change in the peak shape of CsA was investigated with loading amounts of 5, 25, 50, 100, and 500 mg. With an increase in sample loading, the peak shape of CsA in both mobile phases changed from symmetric to tailing, and the retention time reduced. Therefore, it is necessary to focus on the removal of impurities that were eluted before CsA during the purification. In addition, the peak shapes of CsA in methanol-water and acetonitrile-water were similar in the test
ISSN:1000-8713
DOI:10.3724/SP.J.1123.2021.01045