Improvement of laccase activity by silencing PacC in Ganoderma lucidum

• Published in: World journal of microbiology & biotechnology 2022-02, Vol.38 (2), p.32-32, Article 32
• Main Authors: Zhu, Jing, Song, Shuqi, Lian, Lindan, Shi, Liang, Ren, Ang, Zhao, Mingwen
• Format: Article
• Language: English
• Subjects: Acidification; Applied Microbiology; Biochemistry; Biomass; Biomedical and Life Sciences; Biotechnology; Environmental Engineering/Biotechnology; Enzyme Assays; Enzymes; Fermentation; Fungal Proteins - genetics; Fungal Proteins - metabolism; Ganoderma lucidum; Gene Expression Regulation, Fungal; Gene Silencing; Genes; Genes, Fungal - genetics; Genomes; Hydrogen-Ion Concentration; Kinetics; Laccase; Laccase - genetics; Laccase - metabolism; Life Sciences; Lignin; Lignocellulose; Microbiology; Mushrooms; Nucleotide sequence; Original Paper; pH effects; Protein folding; Proteins; Regulatory mechanisms (biology); Regulatory sequences; Reishi - enzymology; Reishi - genetics; Reishi - metabolism; Transcription; White rot; White rot fungi
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-021-03216-x

Ganoderma lucidum is a representative white-rot fungus that has great potential to degrade lignocellulose biomass. Laccase is recognized as a class of the most important lignin-degrading enzymes in G. lucidum . However, the comprehensive regulatory mechanisms of laccase are still lacking. Based on the genome sequence of G. lucidum , 15 laccase genes were identified and their encoding proteins were analyzed in this study. All of the laccase proteins are predicted to be multicopper oxidases with conserved copper-binding domains. Most laccase proteins were secreted enzymes in addition to Lac14 in which the signal peptide could not be predicted. The activity of all laccases showed the highest level at pH 3.0 or pH 7.0, with total laccase activity of approximately 200 U/mg protein. Silencing PacC resulted in a 5.2 fold increase in laccase activity compared with WT. Five laccase genes ( lac1 , lac6 , lac9 , lac10 and lac14 ) showed an increased transcription levels (approximately 1.5–5.6 fold) in the PacC -silenced strains versus that in WT, while other laccase genes were downregulated or unchanged. The extracellular pH value was about 3.1, which was more acidic in the PacC -silenced strains than in the WT (pH 3.5). Moreover, maintaining the fermentation pH resulted in a downregulation of laccase activity which is induced by silencing PacC . Our findings indicate that in addition to its function in acidification of environmental pH, PacC plays an important role in regulating laccase activity in fungi.