Comparison of two fluorescence quantitative immunochromatographic assays for the detection of amantadine in chicken muscle

[Display omitted] •Introduce and compare two fluorescence quantitative immunoassays for AMD detection.•Provide sensitive, and rapid technical support for AMD screening in food matrices.•Recommend suitable immunochromatographic assays for food residues surveillance. The two sensitive fluorescence qua...

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Bibliographic Details
Published in:Food chemistry 2022-05, Vol.377, p.131931-131931, Article 131931
Main Authors: Pan, Yantong, Wang, Zhaopeng, Duan, Changfei, Dou, Leina, Wen, Kai, Wang, Zhanhui, Yu, Xuezhi, Shen, Jianzhong
Format: Article
Language:English
Subjects:
Amantadine
Amantadine - analysis
Animals
Background fluorescence quenching immunochromatographic assay
Chickens
Fluorescence
Fluorescence quantitative immunochromatographic assays
Food Safety
Humans
Immunoassay
Limit of Detection
Muscles - chemistry
Time-resolved immunoassay
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Summary:[Display omitted] •Introduce and compare two fluorescence quantitative immunoassays for AMD detection.•Provide sensitive, and rapid technical support for AMD screening in food matrices.•Recommend suitable immunochromatographic assays for food residues surveillance. The two sensitive fluorescence quantitative immunochromatographic assays (FQICAs), background fluorescence quenching immunochromatographic assay (bFQICA) and time-resolved fluorescent immunochromatographic assay (TRFICA), play an important role increasingly in rapid detection technology for food safety. Amantadine (AMD), used extensively in virus infections in livestock and poultry, has been prohibited due to hazard concerns over public human health. Therefore, AMD was used as a model molecule in the FQICAs establishment and comparison based on the same bioreagents. The outstanding performance in technical parameters of the two FQICAs indicated that they could provide rapid, precise, reliable technical support for large-scale on-site screening for AMD detection. What’s more, the systematic and comprehensive comparison of the two FQICAs would give useful suggestions for scientists and users in monitoring the harmful compounds.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2021.131931