The potential role of eyestalk in the immunity of Litopenaeus vannamei to Vibrio infection

• Published in: Fish & shellfish immunology 2022-02, Vol.121, p.62-73
• Main Authors: Zhang, Xin, Shi, Jialong, Sun, Yulong, Wang, Yilei, Zhang, Ziping
• Format: Article
• Language: English
• Subjects: Animal Structures - immunology; Animals; Aquaculture; Eyestalk ablation; Hemocytes; Immunity, Innate; Immunoregulation; Litopenaeus vannamei; Penaeidae - genetics; Penaeidae - immunology; RNA-seq; Vibrio Infections; Vibrio parahaemolyticus
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2021.12.057

The X-organ-sinus gland complex (XO-SG) in the eyestalk is an important neuroendocrine regulatory organ of crustaceans such as Litopenaeus vannamei, a prominent aquaculture species. The current study found significant changes in the enzyme activities of ALP, ACP, and T-SOD of hepatopancreatic in response to Vibrio parahaemolyticus exposure following eyestalk ablation, indicating that they were all involved in the immunological regulation of shrimps against V. parahaemolyticus infection. A total of 52,656 unigenes were obtained after RNA-Seq, with an average length of 1036 bp and an N50 of 1847 bp. Subsequently, 1899 eyestalk positive regulation genes (EPRGs), 745 eyestalk negative regulation genes (ENRGs), and 2077 non-eyestalk regulatory genes (NEGs) were identified. KEGG analysis of EPRGs revealed that eyestalk ablation might activate the neuroendocrine-immune (NEI) system. The RNA-Seq data were validated using quantitative real-time PCR (qRT-PCR). The findings suggested that eyestalk ablation might affect the expression of genes involved in the prophenoloxidase-activating system, the TLR signaling pathway, and numerous other immune-related genes in L. vannamei. All of these findings revealed that the eyestalk might have a role in the immune response of L. vannamei. The genes and pathways discovered in this study will help to elucidate the molecular mechanisms of hemocytes’ immune response to V. parahaemolyticus following eyestalk ablation in shrimp, as well as provide the framework for building crustacean immunity theory. •The potential role of eyestalk in the immunity of Litopenaeus vannamei to Vibrio parahaemolyticus infection was reported.•After transcriptome assembly, 52,656 unigenes with an average length of 1035,871 nt were identified.•Transcriptome analysis reveals that eyestalk plays a major role in the immunoregulation of L. vannamei.•Possible mechanism of hemocytes in response to V. parahaemolyticus infection after eyestalk ablation was clarified.