Structure and functional capacity of a benzene‐mineralizing, nitrate‐reducing microbial community

• Published in: Journal of applied microbiology 2022-04, Vol.132 (4), p.2795-2811
• Main Authors: Eziuzor, Samuel C., Corrêa, Felipe B., Peng, Shuchan, Schultz, Júnia, Kleinsteuber, Sabine, Rocha, Ulisses N., Adrian, Lorenz, Vogt, Carsten
• Format: Article
• Language: English
• Subjects: Ammonium; anaerobic benzene degradation; Anaerobiosis; anammox bacteria; Anoxic conditions; Benzene; Benzene - metabolism; Carbon sources; Carboxylation; Degradation; dissimilatory nitrate reduction; Enrichment media; Functional groups; Hydrocarbons; Microbiomes; Microbiota; Microorganisms; Mineralization; Nitrates; Nitrates - metabolism; Organic carbon; Oxidation; Oxidation-Reduction; Peptococcaceae; Peptococcaceae - metabolism; putative anaerobic benzene carboxylase; Relative abundance; Trophic relationships
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.15443

Aims How benzene is metabolized by microbes under anoxic conditions is not fully understood. Here, we studied the degradation pathways in a benzene‐mineralizing, nitrate‐reducing enrichment culture. Methods and results Benzene mineralization was dependent on the presence of nitrate and correlated to the enrichment of a Peptococcaceae phylotype only distantly related to known anaerobic benzene degraders of this family. Its relative abundance decreased after benzene mineralization had terminated, while other abundant taxa—Ignavibacteriaceae, Rhodanobacteraceae and Brocadiaceae—slightly increased. Generally, the microbial community remained diverse despite the amendment of benzene as single organic carbon source, suggesting complex trophic interactions between different functional groups. A subunit of the putative anaerobic benzene carboxylase previously detected in Peptococcaceae was identified by metaproteomic analysis suggesting that benzene was activated by carboxylation. Detection of proteins involved in anaerobic ammonium oxidation (anammox) indicates that benzene mineralization was accompanied by anammox, facilitated by nitrite accumulation and the presence of ammonium in the growth medium. Conclusions The results suggest that benzene was activated by carboxylation and further assimilated by a novel Peptococcaceae phylotype. Significance and impact of the study The results confirm the hypothesis that Peptococcaceae are important anaerobic benzene degraders.