Assessment of Pharmacokinetic Parameters of Daidzein-Containing Nanosuspension and Nanoemulsion Formulations After Oral Administration to Rats

• Published in: European journal of drug metabolism and pharmacokinetics 2022-03, Vol.47 (2), p.247-257
• Main Authors: Demirtürk, Esra, Ugur Kaplan, Afife Büşra, Cetin, Meltem, Akıllıoğlu, Kübra, Dönmez Kutlu, Meltem, Köse, Seda, Aksu, Fazilet
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Biomedicine; Human Physiology; Medical Biochemistry; Original Research Article; Pharmaceutical Sciences/Technology; Pharmacology/Toxicology; Pharmacy
• ISSN: 0378-7966; 2107-0180
• DOI: 10.1007/s13318-021-00746-5

Background and Objectives Daidzein has several biological effects such as antioxidation, anti-inflammation, chemoprevention, and anticancer effects. The aim of this study was to evaluate the impact of nano-formulations (nanoemulsion-NE and nanosuspension-NS) prepared to increase the oral bioavailability of daidzein, a poorly water-soluble isoflavone, on the pharmacokinetic parameters of daidzein in rats. Methods A high-performance liquid chromatography-ultraviolet (HPLC-UV) method was successfully developed for daidzein analysis in rat plasma. The pharmacokinetics studies of the nano-sized formulations, compared to coarse daidzein suspension, were carried out in the rats by a single oral dose at 10 mg/kg ( n  = 6/group). Area under the plasma concentration-time curve from time zero to extrapolation to time infinity (AUC 0–∞) , maximum plasma concentration ( C max ), time to reach maximum plasma concentration ( t max ), and elimination half life ( t 1/2 ) values for coarse daidzein suspension, daidzein-NS, and daidzein-NE were estimated by a non-compartmental analysis. Results The AUC values of daidzein-NE and daidzein-NS were approximately 2.62 and 2.65 times higher than that of coarse daidzein suspension, respectively ( p