Establishment of Galectin-3 Time-resolved Fluoroimmunoassay and its Application in Idiopathic Membranous Nephropathy

The aim of this study was to establish a time-resolved fluorescent immunoassay (TRFIA) for the detection of serum Galectin-3 (Gal-3) and apply this method to evaluate the clinical significance of serum Gal-3 in predicting Idiopathic Membranous Nephropathy (IMN) progression. The Gal-3-TRFIA was estab...

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Bibliographic Details
Published in:Journal of fluorescence 2022-03, Vol.32 (2), p.629-636
Main Authors: Yu, Xiaomei, Chen, Lingli, Lin, Bo, Zhang, Li, Yang, Xue, Liu, Xiaobin, Xia, Pengguo, Liu, Yueming, Zheng, Shaoxiong, Zhou, Xiumei, Wang, Yigang, Qin, Yuan, Wang, liang, Hu, Zhigang, He, Qiang, Huang, Biao
Format: Article
Language:English
Subjects:
Analytical Chemistry
Antibodies
Antibodies - blood
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biomedicine
Biophysics
Biotechnology
Coefficient of variation
Correlation coefficients
Disease Progression
Enzyme-Linked Immunosorbent Assay
Europium
Fluorescence
Fluoroimmunoassay - methods
Fluoroimmunoassaying
Galectin 3 - blood
Galectin 3 - immunology
Glomerulonephritis, Membranous - blood
Glomerulonephritis, Membranous - diagnosis
Humans
Original Article
Prospective Studies
Sensitivity and Specificity
Time Factors
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Summary:The aim of this study was to establish a time-resolved fluorescent immunoassay (TRFIA) for the detection of serum Galectin-3 (Gal-3) and apply this method to evaluate the clinical significance of serum Gal-3 in predicting Idiopathic Membranous Nephropathy (IMN) progression. The Gal-3-TRFIA was established using the double antibody sandwich method, with the capture antibodies coated on a 96-well microplate and the detection antibodies chelated with Europium (III) (Eu 3+ ). Serum Gal-3 was detected in 81 patients with IMN and 123 healthy controls to further evaluate the value of the Gal-3 in staging of IMN. The sensitivity of the Gal-3-TRFIA assay was 0.85 ng/mL, and the detection range was 0.85–1000 ng/mL. The Gal-3 intra-batch and inter-batch coefficients of variation were 3.45% and 5.12%, respectively. The correlation coefficient ( R ) between the Gal-3-TRFIA assay and commercially available enzyme-linked immunosorbent assay kits was 0.83. The serum Gal-3 concentration was higher in patients with IMN (65.57 ± 55.90 ng/mL) compared to healthy controls (16.29 ± 9.91 ng/mL, P 
ISSN:1053-0509
1573-4994
DOI:10.1007/s10895-022-02888-4