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Establishment of Galectin-3 Time-resolved Fluoroimmunoassay and its Application in Idiopathic Membranous Nephropathy
The aim of this study was to establish a time-resolved fluorescent immunoassay (TRFIA) for the detection of serum Galectin-3 (Gal-3) and apply this method to evaluate the clinical significance of serum Gal-3 in predicting Idiopathic Membranous Nephropathy (IMN) progression. The Gal-3-TRFIA was estab...
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| Published in: | Journal of fluorescence 2022-03, Vol.32 (2), p.629-636 |
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| Main Authors: | , , , , , , , , , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c419t-c4818f7d30633b61a4d7ad67cbd60dad1a1c643a38601bb0615592a5608e25333 |
| container_end_page | 636 |
| container_issue | 2 |
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| container_title | Journal of fluorescence |
| container_volume | 32 |
| creator | Yu, Xiaomei Chen, Lingli Lin, Bo Zhang, Li Yang, Xue Liu, Xiaobin Xia, Pengguo Liu, Yueming Zheng, Shaoxiong Zhou, Xiumei Wang, Yigang Qin, Yuan Wang, liang Hu, Zhigang He, Qiang Huang, Biao |
| description | The aim of this study was to establish a time-resolved fluorescent immunoassay (TRFIA) for the detection of serum Galectin-3 (Gal-3) and apply this method to evaluate the clinical significance of serum Gal-3 in predicting Idiopathic Membranous Nephropathy (IMN) progression. The Gal-3-TRFIA was established using the double antibody sandwich method, with the capture antibodies coated on a 96-well microplate and the detection antibodies chelated with Europium (III) (Eu
3+
). Serum Gal-3 was detected in 81 patients with IMN and 123 healthy controls to further evaluate the value of the Gal-3 in staging of IMN. The sensitivity of the Gal-3-TRFIA assay was 0.85 ng/mL, and the detection range was 0.85–1000 ng/mL. The Gal-3 intra-batch and inter-batch coefficients of variation were 3.45% and 5.12%, respectively. The correlation coefficient (
R
) between the Gal-3-TRFIA assay and commercially available enzyme-linked immunosorbent assay kits was 0.83. The serum Gal-3 concentration was higher in patients with IMN (65.57 ± 55.90 ng/mL) compared to healthy controls (16.29 ± 9.91 ng/mL, P |
| doi_str_mv | 10.1007/s10895-022-02888-4 |
| format | article |
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3+
). Serum Gal-3 was detected in 81 patients with IMN and 123 healthy controls to further evaluate the value of the Gal-3 in staging of IMN. The sensitivity of the Gal-3-TRFIA assay was 0.85 ng/mL, and the detection range was 0.85–1000 ng/mL. The Gal-3 intra-batch and inter-batch coefficients of variation were 3.45% and 5.12%, respectively. The correlation coefficient (
R
) between the Gal-3-TRFIA assay and commercially available enzyme-linked immunosorbent assay kits was 0.83. The serum Gal-3 concentration was higher in patients with IMN (65.57 ± 55.90 ng/mL) compared to healthy controls (16.29 ± 9.91 ng/mL, P < 0.0001). In this study, a wide detection range Gal-3-TRFIA assay was developed using lanthanide (Eu
3+
) chelates for the detection of Gal-3 concentrations in serum. Gal-3 concentration is elevated in patients with IMN.</description><identifier>ISSN: 1053-0509</identifier><identifier>EISSN: 1573-4994</identifier><identifier>DOI: 10.1007/s10895-022-02888-4</identifier><identifier>PMID: 35025017</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Analytical Chemistry ; Antibodies ; Antibodies - blood ; Biochemistry ; Biological and Medical Physics ; Biomedical and Life Sciences ; Biomedicine ; Biophysics ; Biotechnology ; Coefficient of variation ; Correlation coefficients ; Disease Progression ; Enzyme-Linked Immunosorbent Assay ; Europium ; Fluorescence ; Fluoroimmunoassay - methods ; Fluoroimmunoassaying ; Galectin 3 - blood ; Galectin 3 - immunology ; Glomerulonephritis, Membranous - blood ; Glomerulonephritis, Membranous - diagnosis ; Humans ; Original Article ; Prospective Studies ; Sensitivity and Specificity ; Time Factors</subject><ispartof>Journal of fluorescence, 2022-03, Vol.32 (2), p.629-636</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022</rights><rights>2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-c4818f7d30633b61a4d7ad67cbd60dad1a1c643a38601bb0615592a5608e25333</citedby><cites>FETCH-LOGICAL-c419t-c4818f7d30633b61a4d7ad67cbd60dad1a1c643a38601bb0615592a5608e25333</cites><orcidid>0000-0003-1883-0670</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35025017$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, Xiaomei</creatorcontrib><creatorcontrib>Chen, Lingli</creatorcontrib><creatorcontrib>Lin, Bo</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Yang, Xue</creatorcontrib><creatorcontrib>Liu, Xiaobin</creatorcontrib><creatorcontrib>Xia, Pengguo</creatorcontrib><creatorcontrib>Liu, Yueming</creatorcontrib><creatorcontrib>Zheng, Shaoxiong</creatorcontrib><creatorcontrib>Zhou, Xiumei</creatorcontrib><creatorcontrib>Wang, Yigang</creatorcontrib><creatorcontrib>Qin, Yuan</creatorcontrib><creatorcontrib>Wang, liang</creatorcontrib><creatorcontrib>Hu, Zhigang</creatorcontrib><creatorcontrib>He, Qiang</creatorcontrib><creatorcontrib>Huang, Biao</creatorcontrib><title>Establishment of Galectin-3 Time-resolved Fluoroimmunoassay and its Application in Idiopathic Membranous Nephropathy</title><title>Journal of fluorescence</title><addtitle>J Fluoresc</addtitle><addtitle>J Fluoresc</addtitle><description>The aim of this study was to establish a time-resolved fluorescent immunoassay (TRFIA) for the detection of serum Galectin-3 (Gal-3) and apply this method to evaluate the clinical significance of serum Gal-3 in predicting Idiopathic Membranous Nephropathy (IMN) progression. The Gal-3-TRFIA was established using the double antibody sandwich method, with the capture antibodies coated on a 96-well microplate and the detection antibodies chelated with Europium (III) (Eu
3+
). Serum Gal-3 was detected in 81 patients with IMN and 123 healthy controls to further evaluate the value of the Gal-3 in staging of IMN. The sensitivity of the Gal-3-TRFIA assay was 0.85 ng/mL, and the detection range was 0.85–1000 ng/mL. The Gal-3 intra-batch and inter-batch coefficients of variation were 3.45% and 5.12%, respectively. The correlation coefficient (
R
) between the Gal-3-TRFIA assay and commercially available enzyme-linked immunosorbent assay kits was 0.83. The serum Gal-3 concentration was higher in patients with IMN (65.57 ± 55.90 ng/mL) compared to healthy controls (16.29 ± 9.91 ng/mL, P < 0.0001). In this study, a wide detection range Gal-3-TRFIA assay was developed using lanthanide (Eu
3+
) chelates for the detection of Gal-3 concentrations in serum. Gal-3 concentration is elevated in patients with IMN.</description><subject>Analytical Chemistry</subject><subject>Antibodies</subject><subject>Antibodies - blood</subject><subject>Biochemistry</subject><subject>Biological and Medical Physics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biophysics</subject><subject>Biotechnology</subject><subject>Coefficient of variation</subject><subject>Correlation coefficients</subject><subject>Disease Progression</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Europium</subject><subject>Fluorescence</subject><subject>Fluoroimmunoassay - methods</subject><subject>Fluoroimmunoassaying</subject><subject>Galectin 3 - blood</subject><subject>Galectin 3 - immunology</subject><subject>Glomerulonephritis, Membranous - blood</subject><subject>Glomerulonephritis, Membranous - diagnosis</subject><subject>Humans</subject><subject>Original Article</subject><subject>Prospective Studies</subject><subject>Sensitivity and Specificity</subject><subject>Time Factors</subject><issn>1053-0509</issn><issn>1573-4994</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kU1PHSEUhklT4_cf6KIhceNm2sPwMbA0xq_E1o2uCTNwezEzMAJjcv-96LU2cdEFcALPeTnJg9A3Aj8IQPczE5CKN9C2dUkpG_YF7RPe0YYpxb7WGjhtgIPaQwc5PwKAkkzuoj3KoeVAun1ULnIx_ejzenKh4LjCV2Z0Q_GhofjeT65JLsfx2Vl8OS4xRT9NS4gmZ7PBJljsS8Zn8zz6wRQfA_YB31gfZ1PWfsC_3NQnE-KS8W83r9Pb_eYI7azMmN3x-3mIHi4v7s-vm9u7q5vzs9tmYESVuksiV52lICjtBTHMdsaKbuitAGssMWQQjBoqBZC-B0E4V63hAqRrOaX0EJ1uc-cUnxaXi558Htw4muDqSLoVRHFGW_WKnnxCH-OSQp2uUpRJoTpJKtVuqSHFnJNb6Tn5yaSNJqBfneitE12d6DcnmtWm7-_RSz85-9HyV0IF6BbI9Sn8cenf3_-JfQGAkpei</recordid><startdate>20220301</startdate><enddate>20220301</enddate><creator>Yu, Xiaomei</creator><creator>Chen, Lingli</creator><creator>Lin, Bo</creator><creator>Zhang, Li</creator><creator>Yang, Xue</creator><creator>Liu, Xiaobin</creator><creator>Xia, Pengguo</creator><creator>Liu, Yueming</creator><creator>Zheng, Shaoxiong</creator><creator>Zhou, Xiumei</creator><creator>Wang, Yigang</creator><creator>Qin, Yuan</creator><creator>Wang, liang</creator><creator>Hu, Zhigang</creator><creator>He, Qiang</creator><creator>Huang, Biao</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1883-0670</orcidid></search><sort><creationdate>20220301</creationdate><title>Establishment of Galectin-3 Time-resolved Fluoroimmunoassay and its Application in Idiopathic Membranous Nephropathy</title><author>Yu, Xiaomei ; Chen, Lingli ; Lin, Bo ; Zhang, Li ; Yang, Xue ; Liu, Xiaobin ; Xia, Pengguo ; Liu, Yueming ; Zheng, Shaoxiong ; Zhou, Xiumei ; Wang, Yigang ; Qin, Yuan ; Wang, liang ; Hu, Zhigang ; He, Qiang ; Huang, Biao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-c4818f7d30633b61a4d7ad67cbd60dad1a1c643a38601bb0615592a5608e25333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Analytical Chemistry</topic><topic>Antibodies</topic><topic>Antibodies - blood</topic><topic>Biochemistry</topic><topic>Biological and Medical Physics</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biophysics</topic><topic>Biotechnology</topic><topic>Coefficient of variation</topic><topic>Correlation coefficients</topic><topic>Disease Progression</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Europium</topic><topic>Fluorescence</topic><topic>Fluoroimmunoassay - methods</topic><topic>Fluoroimmunoassaying</topic><topic>Galectin 3 - blood</topic><topic>Galectin 3 - immunology</topic><topic>Glomerulonephritis, Membranous - blood</topic><topic>Glomerulonephritis, Membranous - diagnosis</topic><topic>Humans</topic><topic>Original Article</topic><topic>Prospective Studies</topic><topic>Sensitivity and Specificity</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, Xiaomei</creatorcontrib><creatorcontrib>Chen, Lingli</creatorcontrib><creatorcontrib>Lin, Bo</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Yang, Xue</creatorcontrib><creatorcontrib>Liu, Xiaobin</creatorcontrib><creatorcontrib>Xia, Pengguo</creatorcontrib><creatorcontrib>Liu, Yueming</creatorcontrib><creatorcontrib>Zheng, Shaoxiong</creatorcontrib><creatorcontrib>Zhou, Xiumei</creatorcontrib><creatorcontrib>Wang, Yigang</creatorcontrib><creatorcontrib>Qin, Yuan</creatorcontrib><creatorcontrib>Wang, liang</creatorcontrib><creatorcontrib>Hu, Zhigang</creatorcontrib><creatorcontrib>He, Qiang</creatorcontrib><creatorcontrib>Huang, Biao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of fluorescence</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yu, Xiaomei</au><au>Chen, Lingli</au><au>Lin, Bo</au><au>Zhang, Li</au><au>Yang, Xue</au><au>Liu, Xiaobin</au><au>Xia, Pengguo</au><au>Liu, Yueming</au><au>Zheng, Shaoxiong</au><au>Zhou, Xiumei</au><au>Wang, Yigang</au><au>Qin, Yuan</au><au>Wang, liang</au><au>Hu, Zhigang</au><au>He, Qiang</au><au>Huang, Biao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of Galectin-3 Time-resolved Fluoroimmunoassay and its Application in Idiopathic Membranous Nephropathy</atitle><jtitle>Journal of fluorescence</jtitle><stitle>J Fluoresc</stitle><addtitle>J Fluoresc</addtitle><date>2022-03-01</date><risdate>2022</risdate><volume>32</volume><issue>2</issue><spage>629</spage><epage>636</epage><pages>629-636</pages><issn>1053-0509</issn><eissn>1573-4994</eissn><abstract>The aim of this study was to establish a time-resolved fluorescent immunoassay (TRFIA) for the detection of serum Galectin-3 (Gal-3) and apply this method to evaluate the clinical significance of serum Gal-3 in predicting Idiopathic Membranous Nephropathy (IMN) progression. The Gal-3-TRFIA was established using the double antibody sandwich method, with the capture antibodies coated on a 96-well microplate and the detection antibodies chelated with Europium (III) (Eu
3+
). Serum Gal-3 was detected in 81 patients with IMN and 123 healthy controls to further evaluate the value of the Gal-3 in staging of IMN. The sensitivity of the Gal-3-TRFIA assay was 0.85 ng/mL, and the detection range was 0.85–1000 ng/mL. The Gal-3 intra-batch and inter-batch coefficients of variation were 3.45% and 5.12%, respectively. The correlation coefficient (
R
) between the Gal-3-TRFIA assay and commercially available enzyme-linked immunosorbent assay kits was 0.83. The serum Gal-3 concentration was higher in patients with IMN (65.57 ± 55.90 ng/mL) compared to healthy controls (16.29 ± 9.91 ng/mL, P < 0.0001). In this study, a wide detection range Gal-3-TRFIA assay was developed using lanthanide (Eu
3+
) chelates for the detection of Gal-3 concentrations in serum. Gal-3 concentration is elevated in patients with IMN.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>35025017</pmid><doi>10.1007/s10895-022-02888-4</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-1883-0670</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Analytical Chemistry Antibodies Antibodies - blood Biochemistry Biological and Medical Physics Biomedical and Life Sciences Biomedicine Biophysics Biotechnology Coefficient of variation Correlation coefficients Disease Progression Enzyme-Linked Immunosorbent Assay Europium Fluorescence Fluoroimmunoassay - methods Fluoroimmunoassaying Galectin 3 - blood Galectin 3 - immunology Glomerulonephritis, Membranous - blood Glomerulonephritis, Membranous - diagnosis Humans Original Article Prospective Studies Sensitivity and Specificity Time Factors |
| title | Establishment of Galectin-3 Time-resolved Fluoroimmunoassay and its Application in Idiopathic Membranous Nephropathy |
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