Photolithographic Fabrication of Semi 3‑D Microstructures Composed of Flexible Hydrogel Sheet for in Vivo-like Cell Culture System

Insufficient reliability of current drug screening by cell-based assay has been one of the factors in the poor success rate in drug development. To improve the situation, we proposed a cell culture system using semi 3-D hydrogel microstructures as cell culture scaffolds. Because of its flexibility a...

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Bibliographic Details
Published in:ACS applied bio materials 2019-10, Vol.2 (10), p.4129-4133
Main Authors: Tsuyukubo, Atsushi, Sumaru, Kimio, Kanamori, Toshiyuki
Format: Article
Language:English
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Summary:Insufficient reliability of current drug screening by cell-based assay has been one of the factors in the poor success rate in drug development. To improve the situation, we proposed a cell culture system using semi 3-D hydrogel microstructures as cell culture scaffolds. Because of its flexibility and permeability, the microstructure was expected to enhance the physiological function of cells. We developed a simple method of fabricating the unique hydrogel microstructures composed of hydroxypropyl cellulose through photolithography. Functionalization with poly­(styrene-co-maleic anhydride) gained their cell adhesion, and it was demonstrated that several kinds of cells were incubated on the cell culture scaffolds.
ISSN:2576-6422
2576-6422
DOI:10.1021/acsabm.9b00656