Isolation of purple nonsulfur bacteria from the digestive tract of ayu (Plecoglossus altivelis)

Purple nonsulfur bacteria (PNSB) reportedly have probiotic effects in fish, but whether they are indigenous in the digestive tract of fish is a question that requires answering. We attempted to isolate PNSB from the digestive tract of ayu (Plecoglossus altivelis) from the Kuma River (Kumamoto, Japan...

Full description

Saved in:
Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2022-02, Vol.86 (3), p.407-412
Main Authors: Koga, Aoi, Yamasaki, Takumi, Hayashi, Shuhei, Yamamoto, Shinjiro, Miyasaka, Hitoshi
Format: Article
Language:English
Subjects:
Animals
Gastrointestinal Tract - microbiology
Osmeriformes - microbiology
Phylogeny
Rhodopseudomonas - classification
Rhodopseudomonas - genetics
Rhodopseudomonas - isolation & purification
Rivers - microbiology
RNA, Ribosomal, 16S - genetics
Sodium Chloride - pharmacology
Soil Microbiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Purple nonsulfur bacteria (PNSB) reportedly have probiotic effects in fish, but whether they are indigenous in the digestive tract of fish is a question that requires answering. We attempted to isolate PNSB from the digestive tract of ayu (Plecoglossus altivelis) from the Kuma River (Kumamoto, Japan) and successfully isolated 12 PNSB strains. All the isolated PNSB belonged to the genus Rhodopseudomonas. Five Rhodopseudomonas strains were also isolated from the soil samples collected along the Kuma River. The phylogenetic tree based on the partial sequence of pufLM gene indicated that the PNSB from ayu and soil were similar. The effects of NaCl concentration in growth medium on growth were also compared between the PNSB from ayu and soil. The PNSB from ayu showed a better growth performance at a higher NaCl concentration, suggesting that the intestinal tract of ayu, a euryhaline fish, might provide suitable environment for halophilic microorganisms.
ISSN:1347-6947
1347-6947
DOI:10.1093/bbb/zbac001