A Chemically Well-Defined, Self-Assembling 3D Substrate for Long-Term Culture of Human Pluripotent Stem Cells

Clinical applications of human pluripotent stem cells (PSCs) are limited by the lack of chemically well-defined scaffolds for cell expansion, differentiation, and implantation. In this study, we systematically screened various self-assembling hexapeptides to identify the best matrix for long-term 3D...

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Bibliographic Details
Published in:ACS applied bio materials 2019-04, Vol.2 (4), p.1406-1412
Main Authors: Loo, Yihua, Chan, Yun S, Szczerbinska, Iwona, Tan, Bobby C. P, Wan, Andrew C. A, Ng, Huck H, Hauser, Charlotte A. E
Format: Article
Language:English
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Summary:Clinical applications of human pluripotent stem cells (PSCs) are limited by the lack of chemically well-defined scaffolds for cell expansion, differentiation, and implantation. In this study, we systematically screened various self-assembling hexapeptides to identify the best matrix for long-term 3D PSC culture. Lysine-containing Ac-ILVAGK-NH2 hydrogels maintained best the pluripotency of human embryonic and induced PSCs even after 30 passages. This peptide matrix is also compatible with the use of xeno-free and defined differentiation media. By exploiting its stimuli-responsive sol–gel transition, arrays of encapsulated PSCs can be bioprinted for large-scale cell expansion and derivation of miniaturized organoid cultures for high-throughput screening.
ISSN:2576-6422
2576-6422
DOI:10.1021/acsabm.8b00686