A Chemically Well-Defined, Self-Assembling 3D Substrate for Long-Term Culture of Human Pluripotent Stem Cells

Clinical applications of human pluripotent stem cells (PSCs) are limited by the lack of chemically well-defined scaffolds for cell expansion, differentiation, and implantation. In this study, we systematically screened various self-assembling hexapeptides to identify the best matrix for long-term 3D...

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Published in:ACS applied bio materials 2019-04, Vol.2 (4), p.1406-1412
Main Authors: Loo, Yihua, Chan, Yun S, Szczerbinska, Iwona, Tan, Bobby C. P, Wan, Andrew C. A, Ng, Huck H, Hauser, Charlotte A. E
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cited_by cdi_FETCH-LOGICAL-a370t-57c6116d651055b8c75cca6041a94a52e0a2d873ab5f8228b407e5802b65683a3
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container_title ACS applied bio materials
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creator Loo, Yihua
Chan, Yun S
Szczerbinska, Iwona
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Ng, Huck H
Hauser, Charlotte A. E
description Clinical applications of human pluripotent stem cells (PSCs) are limited by the lack of chemically well-defined scaffolds for cell expansion, differentiation, and implantation. In this study, we systematically screened various self-assembling hexapeptides to identify the best matrix for long-term 3D PSC culture. Lysine-containing Ac-ILVAGK-NH2 hydrogels maintained best the pluripotency of human embryonic and induced PSCs even after 30 passages. This peptide matrix is also compatible with the use of xeno-free and defined differentiation media. By exploiting its stimuli-responsive sol–gel transition, arrays of encapsulated PSCs can be bioprinted for large-scale cell expansion and derivation of miniaturized organoid cultures for high-throughput screening.
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title A Chemically Well-Defined, Self-Assembling 3D Substrate for Long-Term Culture of Human Pluripotent Stem Cells
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