Solid-like Dynamic Nuclear Polarization Observed in the Fluid Phase of Lipid Bilayers at 9.4 T

Dynamic nuclear polarization (DNP) is a powerful method to enhance NMR sensitivity. Much progress has been achieved recently to optimize DNP performance at high magnetic fields in solid-state samples, mostly by utilizing the solid or the cross effect. In liquids, only the Overhauser mechanism is act...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2022-01, Vol.144 (3), p.1164-1168
Main Authors: Kuzhelev, Andrei A, Dai, Danhua, Denysenkov, Vasyl, Prisner, Thomas F
Format: Article
Language:English
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Summary:Dynamic nuclear polarization (DNP) is a powerful method to enhance NMR sensitivity. Much progress has been achieved recently to optimize DNP performance at high magnetic fields in solid-state samples, mostly by utilizing the solid or the cross effect. In liquids, only the Overhauser mechanism is active, which exhibits a DNP field profile matching the EPR line shape of the radical, distinguishable from other DNP mechanisms. Here, we observe DNP enhancements with a field profile indicative of the solid effect and thermal mixing at ∼320 K and a magnetic field of 9.4 T in the fluid phase of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) lipid bilayers doped with the radical BDPA (1,3-bis­(diphenylene)-2-phenylallyl). This interesting observation might open up new perspectives for DNP applications in macromolecular systems at ambient temperatures.
ISSN:0002-7863
1520-5126
DOI:10.1021/jacs.1c12837