The effect of nonylphenol exposure on the stimulation of melanomacrophage centers, estrogen and testosterone level, and ERα gene expression in goldfish

• Published in: Comparative biochemistry and physiology. Toxicology & pharmacology 2022-04, Vol.254, p.109270-109270, Article 109270
• Main Authors: Ardeshir, Rashid Alijani, Rastgar, Sara, Salati, Amir Parviz, Zabihi, Ebrahim, Movahedinia, Abdolali, Feizi, Farideh
• Format: Article
• Language: English
• Subjects: Endocrine disrupter; Estrogen receptor; Melanomacrophage center; Nonylphenol
• ISSN: 1532-0456; 1878-1659
• DOI: 10.1016/j.cbpc.2022.109270

The present study tried to measure the formation of melanomacrophage centers (MMCs) in various organs of male and female goldfish exposed to nonylphenol (NP) and aimed to assess its relationship with the main sexual hormones, estrogen receptor expression, and the pigment content of the MMCs. Immature goldfish were exposed to 10−6 and 10−7 M NP for 25 days. After obtaining blood for measuring testosterone and estrogen (E2) levels, tissue samples were collected from various organs for histological studies, quantifying pigments using ImageJ software and chemical analysis, and measuring ERα gene expression. Results showed that the order of forming MMCs in various organs exposed to NP was liver > spleen > kidney, and the order of ERα gene expression was liver > testes > spleen > kidney in the male, and liver > spleen > kidney > ovaries in the female. Among the three pigments present in MMCs after exposure to the two doses of NP, melanin was more obvious (especially in the liver) and increased mostly in a dose-dependent manner in both sexes (especially in the male). Chemical analyses confirmed these results. Measurement of testosterone and E2 level in male and female goldfish showed that NP had more effect on the concentration of these hormones in male fish, indicating more endocrine-disrupting potential of NP against the male fish. Generally, the increase of melanin content of melanomacrophage centers coincided with the increase of ERα gene expression and decrease of testosterone level in goldfish after exposure to NP. [Display omitted] •NP had more endocrine-disrupting potential against the male fish.•Melanin increased mostly in a dose-dependent manner.•The order of forming MMCs in goldfish after exposure to NP was liver > spleen > kidney.•The order of ERα gene expression was liver > testes > spleen >kidney in the male, and liver > spleen > kidney > ovaries in the female.•The increase of melanin content of MMCs coincided with the increase of ERα gene expression and decrease of testosterone level.