A review on colorimetric assays for DNA virus detection

• Published in: Journal of virological methods 2022-03, Vol.301, p.114461-114461, Article 114461
• Main Authors: Abdolhosseini, Mansoreh, Zandsalimi, Farshid, Moghaddam, Fahimeh Salasar, Tavoosidana, Gholamreza
• Format: Article
• Language: English
• Subjects: Colorimetric assay; CRISPR/Cas; Lateral flow; Loop-mediated isothermal amplification; Nanoparticle
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/j.jviromet.2022.114461

•Colorimetric assays are preferred for their faster detection, cheaper cost, and high sensitivity and specificity.•Detecting by both the naked eye and optical detectors, optical properties of nanoparticles can be utilized for colorimetric methods.•Amplification-based colorimetric method has potential advantages over common PCR-based techniques for rapidity, cost, scale, or portability.•CRISPR/Cas is a powerful, precise, rapid answering, and ultra-sensitive platform for detecting various pathogens.•Lateral flow assay has been the most successful among point of care tests. Early detection is one of the ways to deal with DNA virus widespread prevalence, and it is necessary to know new diagnostic methods and techniques. Colorimetric assays are one of the most advantageous methods in detecting viruses. These methods are based on color change, which can be seen either with the naked eye or with special devices. The aim of this study is to introduce and evaluate effective colorimetric methods based on amplification, nanoparticle, CRISPR/Cas, and Lateral flow in the diagnosis of DNA viruses and to discuss the effectiveness of each of the updated methods. Compared to the other methods, colorimetric assays are preferred for faster detection, high efficiency, cheaper cost, and high sensitivity and specificity. It is expected that the spread of these viruses can be prevented by identifying and developing new methods.