Polydopamine nanoparticle-mediated, click chemistry triggered, microparticle-counting immunosensor for the sensitive detection of ochratoxin A

A rapid and accurate detection method is needed for the quantitation of ochratoxin A in agricultural products due to its high toxicity. A microparticle-counting immunosensor based on polydopamine nanoparticle-mediated click chemistry was established for the highly-sensitive detection of ochratoxin A...

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Bibliographic Details
Published in:Journal of hazardous materials 2022-04, Vol.428, p.128206-128206, Article 128206
Main Authors: Chen, Rui, Dong, Yongzhen, Hong, Feng, Zhang, Xiya, Wang, Xiaohong, Wang, Jia, Chen, Yiping
Format: Article
Language:English
Subjects:
Biosensing Techniques
Click Chemistry
Copper
Cu(I)-catalyzed azide-alkyne cycloaddition
Immunoassay
Indoles
Nanoparticles
Ochratoxin A
Ochratoxins
Particle counting
Polydopamine nanoparticles
Polymers
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Summary:A rapid and accurate detection method is needed for the quantitation of ochratoxin A in agricultural products due to its high toxicity. A microparticle-counting immunosensor based on polydopamine nanoparticle-mediated click chemistry was established for the highly-sensitive detection of ochratoxin A. Polydopamine nanoparticles with good biocompatibility and a strong metal-chelating ability were synthesized and conjugated with the antibody. The Coupled compounds were then used as an immune carrier to change the Cu2+ concentration via an immuno-reaction. Some of the remaining Cu2+ ions were reduced to Cu+ ions, which caused azide-polystyrene microspheres and alkyne-polystyrene microspheres to aggregate via a Cu+ ion-mediated click reaction. Particle counting was used to distinguish changes in the sizes of the polystyrene microspheres from dispersed to aggregated to detect ochratoxin A. It showed a wide linear detection range of 0.5–800 ng/mL, and a detection limit of 0.2 ng/mL. This assay provides an attractive analytical tool for the accurate detection of trace targets in complex samples. [Display omitted] •Polydopamine nanoparticle can excellently couple antibodies and chelate Cu2+ ions.•Click ligand-modified polystyrene microparticles are efficient signal probes.•Microparticle-counting immunosensor is an enzyme-free sensor to detect ochratoxin A.
ISSN:0304-3894
1873-3336
DOI:10.1016/j.jhazmat.2021.128206