Self-ligating brackets exhibit accumulation of high levels of periodontopathogens in gingival crevicular fluid

[Abstract] Different types of brackets seem to influence the disruption of the oral microbial environment. Therefore, the aim of this study was to evaluate the influence of self-ligating brackets on the gingival crevicular fluid levels of the putative periodontal pathogens Aggregatibacter actinomyce...

Full description

Saved in:
Bibliographic Details
Published in:Odontology 2022-07, Vol.110 (3), p.460-466
Main Authors: Bergamo, Ana Zilda Nazar, Casarin, Renato Corrêa Viana, do Nascimento, Cássio, Matsumoto, Mírian Aiko Nakane, de Carvalho, Fabrício Kitazono, da Silva, Raquel Assed Bezerra, da Silva, Léa Assed Bezerra, Nelson-Filho, Paulo
Format: Article
Language:English
Subjects:
Aggregatibacter actinomycetemcomitans
Dentistry
Deoxyribonucleic acid
DNA
Elastomers
Female
Gingiva
Gingival Crevicular Fluid
Gum disease
Humans
Male
Medicine
Microbiota
Oral and Maxillofacial Surgery
Original Article
Orthodontic Brackets - microbiology
Orthodontics
Periodontal Diseases
Porphyromonas gingivalis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:[Abstract] Different types of brackets seem to influence the disruption of the oral microbial environment. Therefore, the aim of this study was to evaluate the influence of self-ligating brackets on the gingival crevicular fluid levels of the putative periodontal pathogens Aggregatibacter actinomycetemcomitans sorotype a (Aaa), Tannerella forsythia, Fusobacterium nucleatum, and Porphyromonas gingivalis. Sixty samples of crevicular fluid of twenty patients (11 boys and 9 girls) were analysed at baseline (T0) and after 30 (T1) and 60 (T2) days of bonding of the self-ligating (In-Ovation(R) R, Dentsply, GAC or SmartClip(TM), 3 M Unitek, Monrovia, CA, USA) and of one conventional bracket (Gemini(TM), 3 M Unitek, Monrovia, CA, USA) used with elastomeric ligatures. Total DNA from samples was extracted using CTAB-DNA precipitation method and Real-time PCR was performed to analyse bacterial level. Non-parametric Friedman and Wilcoxon tests were used for data analysis (p value of
ISSN:1618-1247
1618-1255
DOI:10.1007/s10266-021-00677-2