In vitro bioactivity of 3D microstructure hydroxyapatite/collagen based‐egg white as an antibacterial agent

• Published in: Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2022-06, Vol.110 (6), p.1412-1424
• Main Authors: Patty, Diana Julaidy, Nugraheni, Ari Dwi, Ana, Ika Dewi, Yusuf, Yusril
• Format: Article
• Language: English
• Subjects: Annealing furnaces; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antibacterial agents; Biocompatibility; Biological activity; Biomedical materials; Cell density; Cell viability; Collagen; Collagen - chemistry; Collagen - pharmacology; Compressive strength; Crystal structure; Crystallinity; Durapatite - chemistry; Durapatite - pharmacology; Egg White; Eggs; Fourier transforms; Heat treatment; Heat treatments; Humans; Hydroxyapatite; in vitro; Infrared spectra; Infrared spectroscopy; Materials research; Materials science; Nanocomposites; Nutrients; Periodontitis; Physicochemical properties; Precipitation heat treatment; Scaffolds; Spectroscopy, Fourier Transform Infrared; Spectrum analysis; Staphylococcus aureus; Synthesis; X-Ray Diffraction
• ISSN: 1552-4973; 1552-4981
• DOI: 10.1002/jbm.b.35009

The present study aims to design 3D scaffold hydroxyapatite (HA)/collagen (Coll) based egg‐white (EW) as antibacterial properties. The calcium source in HA synthesis derived from the Pinctada maxima shell cultivated on Bali Island has proven biocompatibility, and the compressive strength exceeded human bone. HA synthesis by precipitation with heat treatment in oven‐dried at 80°C (HA‐80) and annealed at 900°C (HA‐900), has crystallinity 48% and 85%, respectively, were used for scaffold design. The physicochemical properties of X‐ray diffractometer spectra showed that increasing temperature affected the crystallinity and HA phase formed. Furthermore, the crystal structure of HA changed in nanocomposite due to the substitution of Coll and EW, and the Fourier transform infrared spectroscopy spectra confirmed that the absorption peak of the phosphate group (1027–1029 cm−1) decreased intensity, presumably by protein binding of EW and Coll. The cell viability of HA/Coll/EW in 24, 48, and 72 h incubation period was 112.34 ± 4.36, 104.89 ± 3.41, 72.88 ± 6.85, respectively. The decreases of cell viability due to high cell density and reduced nutrients in wells. Antibacterial activity of HA/Col/EW exhibited a strong zone of inhibition against bacteria causing periodontitis; Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, and Staphylococcus aureus.