Flavonostilbenes Isolated from the Stems of Rhamnoneuron balansae as Potential SIRT1 Activators

• Published in: Journal of natural products (Washington, D.C.) D.C.), 2022-01, Vol.85 (1), p.70-82
• Main Authors: Cho, Hyo-Moon, Zhang, Mi, Park, Eun-Jin, Lee, Ba-Wool, Park, Yeon-Joo, Kim, Hyun-Woo, Pham, Ha-Thanh-Tung, Chin, Young-Won, Oh, Won-Keun
• Format: Article
• Language: English
• Subjects: Adenylate Kinase - metabolism; Animals; Carbon-13 Magnetic Resonance Spectroscopy; Enzyme Activation; Humans; NAD - metabolism; Plant Stems - chemistry; Proton Magnetic Resonance Spectroscopy; Sirtuin 1 - drug effects; Spectrometry, Mass, Electrospray Ionization; Stilbenes - isolation & purification; Stilbenes - pharmacology; Thymelaeaceae - chemistry
• ISSN: 0163-3864; 1520-6025
• DOI: 10.1021/acs.jnatprod.1c00689

The cumulative effects of cell damage result in aging, which gradually decreases human function in various aspects and leads to multiple age-related chronic diseases. To overcome the adverse effects of aging, silent mating type information regulation 2 homologue (SIRT1) activators are promising bioactive compounds that mimic calorie restriction to improve quality of life and prevent aging. In this study, 11 new flavonostilbenes (1–11) and three known compounds (12–14) were purified from stems of Rhamnoneuron balansae. The structures of the new compounds were determined using extensive data from spectroscopic methods, including NMR and HRESIMS. Their absolute configurations were deduced by ECD calculations with coupling constant analysis. All of the isolated new compounds (1–11) were evaluated for their effects on SIRT1 deacetylase activity, the NAD+/NADH ratio, and the AMP-activated protein kinase activation level in cell-based assays. The results showed that rhamnoneuronal D (1) exhibits promising biological activity in several in vitro models related to SIRT1 and suggest it is a potential natural-product-based antiaging agent.