A Versatile Platform for Sensitive and Label-Free Identification of Biomarkers through an Exo-III-Assisted Cascade Signal Amplification Strategy

The development of a versatile and sensitive analytical biomarker detection platform is important for both early diagnosis and treatment of diseases. In the present study, we propose a novel fluorescence-based, ultrasensitive, and label-free biomarker detection platform. This platform relies on a fl...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2022-02, Vol.94 (4), p.2298-2304
Main Authors: Zhao, Xianxian, Yuan, Ye, Liu, Xiaoya, Mao, Fajiang, Xu, Ge, Liu, Qingzhong
Format: Article
Language:English
Subjects:
Amplification
Antigens
Antitumor agents
Biomarkers
Biosensing Techniques
Carcinoembryonic antigen
Chemistry
Diagnosis
Drug development
Exodeoxyribonucleases - metabolism
Fluorescence
Limit of Detection
Multiplexing
Nucleic Acid Amplification Techniques
Peptide mapping
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Summary:The development of a versatile and sensitive analytical biomarker detection platform is important for both early diagnosis and treatment of diseases. In the present study, we propose a novel fluorescence-based, ultrasensitive, and label-free biomarker detection platform. This platform relies on a flexible probe design compatible for multiple biomarker identification and Exo-III enzyme-triggered cascade signal amplification. We have validated that this label-free platform exhibits high sensitivity and specificity. Indeed, this platform exhibited brilliant analytical performance in qualifying a carcinoembryonic antigen and small extracellular vesicles (sEVs). It also shows excellent capability in multiplexing mapping of surface proteins of various cancer-derived sEVs. Therefore, we believe that the proposed sensing platform has great potential for clinical diagnosis and anticancer drug development.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.1c05012