Nostoc flagelliforme capsular polysaccharides from different culture conditions improve hyperlipidemia and regulate intestinal flora in C57BL/6J mice to varying degrees

• Published in: International journal of biological macromolecules 2022-03, Vol.202, p.224-233
• Main Authors: Li, Jian, Shen, Shi-gang, Han, Cheng-feng, Liu, Shu-ting, Zhang, Le-le, Chen, Ni, Jia, Shi-ru, Han, Pei-pei
• Format: Article
• Language: English
• Subjects: Animals; Culture conditions; Gastrointestinal Microbiome; Hyperlipidemias - drug therapy; Hypolipidemia; Intestinal flora; Lipid metabolism; Mice; Mice, Inbred C57BL; Nostoc; Nostoc flagelliforme capsular polysaccharides; Polysaccharides - pharmacology
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2022.01.034

Two capsular polysaccharides (WL-CPS-1 and GLU-CPS-1) purified from Nostoc flagelliforme under normal and mixotrophic culture conditions were used to investigate the hypolipidemic activity and effect on intestinal flora in C57BL/6J mice respectively. Their molecular weight and monosaccharide composition have been determined in previous studies. They both improved the lipid level by affecting the expression of lipid metabolism genes. They down-regulated the TNF-α and IL-1β levels in serum and up-regulated the activity of antioxidant enzymes in liver thus decreased the atherosclerosis index and MDA content. They up-regulated the short chain fatty acids (SCFAs) synthesis. They decreased the abundance of pathogenic bacteria and increased the abundance of probiotics positively correlated with SCFAs. Compared with WL-CPS-1, GLU-CPS-1 exhibited higher in vivo activity and enriched Odoribacter and Alloprevotella correlating with the gene expression of lipid metabolism, suggesting that the bioactivity of polysaccharides could be regulated by culture conditions. These findings contributed to application of N. flagelliforme polysaccharides with higher activity in hypolipidemia by adjusting culture conditions. [Display omitted] •NFCPS had significant hypolipidemic bioactivity.•They changed the composition of intestinal flora in mice.•SCFAs and lipid metabolism genes were correlated with the intestinal flora changed.•Culture conditions affected the hypolipidemic activity and effect on intestinal flora of NFCPS.