Development of quantitative assay for simultaneous measurement of purine metabolites and creatinine in biobanked urine by liquid chromatography-tandem mass spectrometry

Purine metabolism is essential for all known living creatures, including humans in whom elevated serum concentration of purine break-down product uric acid (UA) is probably an independent risk factor for mortality, type 2 diabetes and cardiovascular events. An automated multiplex assay that measures...

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Bibliographic Details
Published in:Scandinavian journal of clinical and laboratory investigation 2022-02, Vol.82 (1), p.37-49
Main Authors: Svistounov, Dmitri, Solbu, Marit D., Jenssen, Trond G., Mathisen, Ulla Dorte, Hansen, Terkel, Elgstøen, Katja Benedikte Prestø, Zykova, Svetlana N.
Format: Article
Language:English
Subjects:
allantoin
Allantoin - urine
biological specimen bank
Chromatography, High Pressure Liquid - methods
Chromatography, Liquid
creatinine
Creatinine - urine
Diabetes Mellitus, Type 2
Humans
hydrophilic interaction
hypoxanthine
Hypoxanthine - urine
Liquid chromatography
mass spectroscopy
Purines
reference ranges
solubility
Tandem Mass Spectrometry
Uric Acid
urine
xanthine
Xanthine - urine
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Summary:Purine metabolism is essential for all known living creatures, including humans in whom elevated serum concentration of purine break-down product uric acid (UA) is probably an independent risk factor for mortality, type 2 diabetes and cardiovascular events. An automated multiplex assay that measures several purine metabolites could therefore prove useful in many areas of medical, veterinary and biological research. The aim of the present work was to develop a sensitive LC-MS/MS method for simultaneous quantitation of xanthine, hypoxanthine, UA, allantoin, and creatinine in biobanked urine samples. This article describes details and performance of the new method studied in 55 samples of human urine. Archival sample preparation and effect of storage conditions on stability of the analytes are addressed. The intra-day and inter-day coefficients of variation were small for all the analytes, not exceeding 1% and 10%, respectively. Measurements of UA and creatinine in biobanked urine showed good agreement with values obtained using routine enzymatic assays on fresh urine. Spearman's correlation coefficients were 0.869 (p 
ISSN:0036-5513
1502-7686
1502-7686
DOI:10.1080/00365513.2021.2015799