Ineffectiveness of TF-Test® and Coproplus® Methods in Strongyloides stercoralis Infection Diagnosis

Purpose S. stercoralis diagnosis is currently performed by parasitological methods, mainly by Baermann–Moraes (BM), although Agar Plate Culture (APC) presents a higher sensitivity. New techniques, such as TF-Test® and Coproplus® have been suggested as more practical alternatives. The aim of this stu...

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Bibliographic Details
Published in:Acta parasitologica 2022-06, Vol.67 (2), p.732-739
Main Authors: Oliveira, Cíntia de Lima, de Souza, Joelma Nascimento, Souza, Alex Bruno da Silva, Barreto, Nilo Manoel Pereira Vieira, Ribeiro, Irlana Dias, Sampaio, Larissa Mota, Araújo, Weslei Almeida Costa, dos Santos, Juliane Silva Batista, Teixeira, Márcia Cristina Aquino, Soares, Neci Matos
Format: Article
Language:English
Subjects:
Animal Systematics/Taxonomy/Biogeography
Biomedical and Life Sciences
Biomedicine
Diagnosis
Ecology
Feces
Infections
Larvae
Medical Microbiology
Microbiology
Original Paper
Parasites
Parasitic diseases
Parasitology
Protozoa
Sensitivity analysis
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Summary:Purpose S. stercoralis diagnosis is currently performed by parasitological methods, mainly by Baermann–Moraes (BM), although Agar Plate Culture (APC) presents a higher sensitivity. New techniques, such as TF-Test® and Coproplus® have been suggested as more practical alternatives. The aim of this study was to evaluate the sensitivity of TF-Test® and Coproplus®, compared with APC, BM and Spontaneous Sedimentation (SS) methods. Methods One-hundred and forty male alcoholic patients, who provided three stools samples collected on alternate days, were included in this study. The most frequently found parasite was S. stercoralis , 20% (28/140), and the most sensitive method was APC, 96.4% (27/28), followed by BM, 89.3% (25/28) and SS, 57.1% (16/28). TF-Test® and Coproplus® presented a sensitivity of 46.4 (13/28) and 39.3% (11/28), respectively. In samples with a parasitic load of 1–10 larvae/g of feces, which occurred in 39.3% (11/28) of the infected patients, both the TF-Test® and Coproplus® methods demonstrated sensitivities of 18.2% (2/11), while APC and BM methods reached a sensitivity of 100% (11/11) ( p  
ISSN:1230-2821
1896-1851
DOI:10.1007/s11686-021-00484-3