Pedobacter segetis sp. nov., a Novel Bacterium Isolated from Soil

A Gram-negative, motile by gliding, rod-shaped, aerobic bacterium, designated SD-b T , was isolated from a soil sample collected on Dokdo Island, South Korea. A polyphasic approach based on phenotypic, phylogenetic, and genomic analyses was used to characterize the new isolate. Phylogenetic analysis...

Full description

Saved in:
Bibliographic Details
Published in:Current microbiology 2022-02, Vol.79 (2), p.71-71, Article 71
Main Authors: Ten, Leonid N., Li, Weilan, Hong, Soo-Min, Kim, Myung Kyum, Lee, Seung-Yeol, Jung, Hee-Young
Format: Article
Language:English
Subjects:
'Open Collection': Novel Prokaryotic Taxa
Biomedical and Life Sciences
Biotechnology
Deoxyribonucleic acid
DNA
DNA, Bacterial - genetics
Fatty acids
Genes
Genomic analysis
Gliding
Hybridization
Life Sciences
Lipids
Microbiology
New species
Nucleotides
Pedobacter - genetics
Phosphatidylethanolamine
Phylogenetics
Phylogeny
Quinones
RNA, Ribosomal, 16S - genetics
rRNA 16S
Soil
Soil bacteria
Soil Microbiology
Soil microorganisms
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A Gram-negative, motile by gliding, rod-shaped, aerobic bacterium, designated SD-b T , was isolated from a soil sample collected on Dokdo Island, South Korea. A polyphasic approach based on phenotypic, phylogenetic, and genomic analyses was used to characterize the new isolate. Phylogenetic analysis of 16S rRNA gene sequence showed that strain SD-b T belonged to the family Sphingobacteriaceae and most closely related to Pedobacter psychrophilus P4487A T (95.9% similarity). The isolate contained MK-7 as the predominant respiratory quinone; its main polar lipid was phosphatidylethanolamine; and the major fatty acids were summed feature 3 (C 16:1 ω 7 c /C 16:1 ω 6 c ; 32.0%), C 15:0 iso (19.1%), C 17:0 iso 3-OH (8.3%), and C 16:0 (8.2%). The draft genome had a length of 3,842,102 bp with a G+C content of 36.0 mol%, predicting 3282 coding sequences, 3 rRNA genes, 3 ncRNAs, and 36 tRNAs genes. The digital DNA–DNA hybridization and average nucleotide identity values between strain SD-b T and P. psychrophilus LMG 29436 T were 22.0% and 78.9%, respectively. The results of phenotypic properties, genotypic distinctiveness, and chemotaxonomic features support the discrimination of SD-b T from its phylogenetic relatives. Pedobacter segetis sp. nov. is therefore proposed with SD-b T (= KCTC 82351 T  = JCM 34283 T ) as the type strain.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-021-02753-w