GABA-mediated inhibition of cadmium uptake and accumulation in apples

GABA, a four-carbon non-protein amino acid, plays an important role in animals and plants. We previously found GABA could alleviate alkali stress in apple seedlings. However, its physiological mechanism under heavy metal cadmium (Cd) stress need to be further studied. Thus, we explored its biologica...

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Published in:Environmental pollution (1987) 2022-05, Vol.300, p.118867-118867, Article 118867
Main Authors: Li, Yuxing, Li, Yunhao, Cui, Yinglian, Xie, Yuanmei, Shi, Yanjiao, Shang, Yueming, Ma, Fengwang, Zhang, Jing, Li, Cuiying
Format: Article
Language:English
Subjects:
Apple
Cadmium
Cadmium - metabolism
Cadmium - toxicity
GABA
gamma-Aminobutyric Acid - metabolism
gamma-Aminobutyric Acid - pharmacology
Malus - genetics
Malus - metabolism
Plant Roots - metabolism
Root tip
Seedlings
Transport
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Summary:GABA, a four-carbon non-protein amino acid, plays an important role in animals and plants. We previously found GABA could alleviate alkali stress in apple seedlings. However, its physiological mechanism under heavy metal cadmium (Cd) stress need to be further studied. Thus, we explored its biological role in response to Cd stress. It was verified that 0.5 mM GABA could effectively alleviate Cd toxicity. Using NMT technique, we found that exogenous GABA could significantly reduce the net Cd2+ fluxes in apple roots, and Cd content was significantly lower than that in roots under Cd stress. Further analysis indicated exogenous GABA could significantly reduce the expression of genes related to the uptake and transport of Cd in apples under Cd stress. In addition, exogenous GABA could significantly increase the content of amino acids in apple roots under Cd stress. GAD is a key enzyme in GABA synthesis, we obtained transgenic apple roots of overexpression MdGAD1. Compared with the control, transgenic roots accumulated less Cd, maintained lower Cd uptake by roots, and lower expression of related transport genes. These results showed that GABA could effectively alleviate Cd toxicity in apple seedlings and provide a new perspective of GABA to alleviate Cd stress. [Display omitted] •We found that GABA could alleviate Cd stress in apple seedlings.•GABA could significantly reduce the uptake and transport of Cd by apple roots.•Overexpression of MdGAD1 reduced the toxicity of Cd in apples.
ISSN:0269-7491
1873-6424
DOI:10.1016/j.envpol.2022.118867