Similarity and difference between aging and puncture‐induced intervertebral disc degeneration

The purpose of our study was to investigate the changes in micromorphology and mechanical properties of intervertebral discs degeneration induced by aging and puncture. Normal group (NG), 2 weeks post‐puncture degeneration group (PDG) and aging degeneration group (ADG) each included 10 rats. Plain f...

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Bibliographic Details
Published in:Journal of orthopaedic research 2022-11, Vol.40 (11), p.2565-2575
Main Authors: He, Shuangjun, Zhang, Yijian, Zhou, Zhangzhe, Shao, Xiaofeng, Chen, Kangwu, Dai, Shouqian, Liang, Ting, Qian, Zhonglai, Luo, Zongping
Format: Article
Language:English
Subjects:
atomic force microscopy
biomechanics
collagen fiber
elastic modulus
intervertebral disc
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Summary:The purpose of our study was to investigate the changes in micromorphology and mechanical properties of intervertebral discs degeneration induced by aging and puncture. Normal group (NG), 2 weeks post‐puncture degeneration group (PDG) and aging degeneration group (ADG) each included 10 rats. Plain film, magnetic resonance imaging, and histological testing were utilized to assess intervertebral disc degeneration. Atomic force microscope was utilized to analyze the microstructure and elastic modulus of the intervertebral disc, while immunohistochemistry was employed to assess alterations in the cell matrix using collagen I, collagen II, matrix metalloproteinase‐3 (MMP‐3), and tumour necrosis factor‐α (TNF‐α). The results showed that the disc height ratio between PDG and ADG decreased. In the PDG and ADG group, histological scores both increased, the gray value of the T2 signal decreased, the proportion of MMP‐3 and TNF‐positive cells in intervertebral disc tissues was higher (p 
ISSN:0736-0266
1554-527X
DOI:10.1002/jor.25281