Zinc oxide nanoparticles attenuate prepubertal exposure to cisplatin- induced testicular toxicity and spermatogenesis impairment in rats

Cisplatin exposure represents a significant fertility problem for childhood cancer. In this study we examined the possible therapeutic role of Zinc oxide nanoparticles (ZnO-NPs) on Cisplatin (Cis) induced impairment in the spermatogenesis initiation during puberty. Seventy-two male rats aged 30 days...

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Published in:Toxicology (Amsterdam) 2022-02, Vol.468, p.153102-153102, Article 153102
Main Authors: Hamam, Eman T., Awadalla, Amira, shokeir, Ahmed A., Aboul-Naga, Amoura M.
Format: Article
Language:English
Subjects:
Animals
Antineoplastic Agents - toxicity
Body Weight
Cisplatin
Cisplatin - toxicity
Comet Assay
Immunohistochemistry
Male
Microscopy, Electron, Transmission
Nanoparticles
Organ Size
Oxidative Stress - drug effects
prepubertal
Rats
Rats, Sprague-Dawley
spermatogenesis
Spermatogenesis - drug effects
testis
Testis - chemistry
Testis - drug effects
Testis - physiology
Testis - ultrastructure
Vimentin - pharmacology
Zinc Oxide - pharmacology
zinc oxide nanoparticles
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Summary:Cisplatin exposure represents a significant fertility problem for childhood cancer. In this study we examined the possible therapeutic role of Zinc oxide nanoparticles (ZnO-NPs) on Cisplatin (Cis) induced impairment in the spermatogenesis initiation during puberty. Seventy-two male rats aged 30 days were distributed into four equal groups: Control group; ZnO-NPs group (intraperitoneal i.p. injected with 5 mg/kg ZnO-NPs once a week for eight weeks); Cis group (i.p. injected with a single dose of 5 mg/kg); ZnO-NPs + Cis group (ZnO-NPs injection 2 hrs before Cis). Each group was subdivided into three groups and was sacrificed 7, 30 and, 60 days after cisplatin induction, which considered prepubertal at 37-day-old, productive at 60-day-old, and completely adult at 90-day-old. Biochemical, molecular, immunohistochemical, and ultrastructural examinations were studied on the testicular tissues and sperm samples. Group treated with Cis showed a decrease in the antioxidant activity and an increase in the reactive oxygen species (ROS), which in turn caused disruption in blood-testis barrier (BTB) proteins in the three different rat ages, and sperm DNA damage in the adult rats compared to control group (p 
ISSN:0300-483X
1879-3185
DOI:10.1016/j.tox.2022.153102