A hybrid oxygen-generating wound dressing based on chitosan thermosensitive hydrogel and decellularized amniotic membrane

Amniotic membrane (AM) has been utilized as a wound dressing extensively. Given the importance of oxygen in wound healing, here we have reported the fabrication and characterization of an oxygen-generating wound dressing based on AM. This construct was composed of H2O2-loaded polylactic acid (PLA) m...

Full description

Saved in:
Bibliographic Details
Published in:Carbohydrate polymers 2022-04, Vol.281, p.119020-119020, Article 119020
Main Authors: Dadkhah Tehrani, Fatemeh, Shabani, Iman, Shabani, Azadeh
Format: Article
Language:English
Subjects:
adhesion
Amnion
Amniotic membrane
Bandages
blood
cell adhesion
cell growth
chitosan
Chitosan - chemistry
Chitosan temperature-sensitive hydrogel
encapsulation
hemolysis
Humans
hydrogels
Hydrogels - chemistry
Hydrogen Peroxide
Oxygen
PLA microparticles
polylactic acid
porosity
thermosensitivity
Wound dressing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Amniotic membrane (AM) has been utilized as a wound dressing extensively. Given the importance of oxygen in wound healing, here we have reported the fabrication and characterization of an oxygen-generating wound dressing based on AM. This construct was composed of H2O2-loaded polylactic acid (PLA) microparticles embedded within a chitosan/β-glycerophosphate (β-GP) thermosensitive hydrogel covered with a layer of decellularized human-AM. The microparticles had a diameter of 4.48 ± 1.8 μm, an encapsulation efficiency of 44.172 ± 4.49%, and generated oxygen for at least 7 days. The hybrid construct was formed at 32.4 ± 2 °C, had a porous structure (84.69 ± 8.34%) with a pore size of 46.72 ± 26.21 μm. The hydrogel/dAM extract was non-toxic after 7 days based on our MTT results, and the final composite supported cell growth and adhesion. This sample had the most negligible blood cell adhesion with less than 5% hemolysis. Our results indicate the proposed structure's desirable biological, chemical, and physical properties as an active wound dressing. [Display omitted]
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2021.119020