Heterologous expression of a polyketide synthase ACRTS2 in Aspergillus oryzae produces host-selective ACR toxins: coproduction of minor metabolites

Previously, we succeeded to produce the core structure of the host-selective ACR toxin (1) on brown leaf spot on rough lemon when the polyketide synthase ACRTS2 gene was heterologously expressed in Aspergillus oryzae (AO). To confirm the production of 1 in AO, the detection limit and suppressing dec...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2022-02, Vol.86 (3), p.287-293
Main Authors: Kotani, Akari, Ozaki, Taro, Takino, Junya, Mochizuki, Susumu, Akimitsu, Kazuya, Minami, Atsushi, Oikawa, Hideaki
Format: Article
Language:English
Subjects:
Aspergillus oryzae - enzymology
Aspergillus oryzae - genetics
Aspergillus oryzae - metabolism
Gene Expression
Mycotoxins - biosynthesis
Mycotoxins - genetics
Mycotoxins - metabolism
Polyketide Synthases - genetics
Polyketide Synthases - metabolism
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Summary:Previously, we succeeded to produce the core structure of the host-selective ACR toxin (1) on brown leaf spot on rough lemon when the polyketide synthase ACRTS2 gene was heterologously expressed in Aspergillus oryzae (AO). To confirm the production of 1 in AO, the detection limit and suppressing decarboxylation were improved, and these efforts led us to conclude the direct production of 1 instead of its decarboxylation product. During this examination, minor ACR-toxin-related metabolites were found. Their structure determination enabled us to propose a decarboxylation mechanism and a novel branching route forming byproducts from the coupling of the dihydropyrone moiety of 1 with the acetaldehyde and kojic acid abundant in AO. The involvement of putative cyclase ACRTS3 in the chain release of linear polyketide was excluded by the coexpression analysis of ACRTS2 and ACRTS3. Taken together, we concluded that the production of 1 in AO is solely responsible for ACRTS2.
ISSN:1347-6947
1347-6947
DOI:10.1093/bbb/zbab214