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Total Ginsenoside Extract from Panax ginseng Enhances Neural Stem Cell Proliferation and Neuronal Differentiation by Inactivating GSK-3β
Objective To study the effects of total ginsenosides (TG) extract from Panax ginseng on neural stem cell (NSC) proliferation and differentiation and their underlying mechanisms. Methods The migration of NSCs after treatment with various concentrations of TG extract (50, 100, or 200 µ g/mL) were moni...
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| Published in: | Chinese journal of integrative medicine 2022-03, Vol.28 (3), p.229-235 |
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| Main Authors: | , , , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c344t-86c6fd87000e0ee07bb7f724c36f5c66d9ef60f3f6c51f576027045556cf58803 |
| container_end_page | 235 |
| container_issue | 3 |
| container_start_page | 229 |
| container_title | Chinese journal of integrative medicine |
| container_volume | 28 |
| creator | Lin, Kai-li Zhang, Ji Chung, Hau-lam Wu, Xin-yi Liu, Bin Zhao, Bo-xin Sze, Stephen Cho-wing Zhou, Ping-zheng Yung, Ken Kin-lam Zhang, Shi-qing |
| description | Objective
To study the effects of total ginsenosides (TG) extract from
Panax ginseng
on neural stem cell (NSC) proliferation and differentiation and their underlying mechanisms.
Methods
The migration of NSCs after treatment with various concentrations of TG extract (50, 100, or 200 µ g/mL) were monitored. The proliferation of NSCs was examined by a combination of cell counting kit-8 and neurosphere assays. NSC differentiation mediated by TG extract was evaluated by Western blotting and immunofluorescence staining to monitor the expression of nestin and microtubule associated protein 2 (MAP2). The GSK-3β/β-catenin pathway in TG-treated NSCs was examined by Western blot assay. The NSCs with constitutively active GSK-3β mutant were made by adenovirus-mediated gene transfection, then the proliferation and differentiation of NSCs mediated by TG were further verified.
Results
TG treatment significantly enhanced NSC migration (
P |
| doi_str_mv | 10.1007/s11655-021-3508-1 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2623329563</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2623329563</sourcerecordid><originalsourceid>FETCH-LOGICAL-c344t-86c6fd87000e0ee07bb7f724c36f5c66d9ef60f3f6c51f576027045556cf58803</originalsourceid><addsrcrecordid>eNp9kc9u1DAQxi0Eon_gAbggH7mkHduxkxzRsixVK6jUcra8znhxldjFTlD7CLxOH4RnqrcpHDl5rO8332i-IeQdgxMG0JxmxpSUFXBWCQltxV6QQ9Z1ooIa-MtSq4aXmskDcpTzDYBsFMjX5GBP16prD8nv6ziZgW58yBhi9j3S9d2UjJ2oS3GklyaYO7p7knd0HX6YYDHTrzin0nY14UhXOAz0MsXBO0xm8jFQE_onJIYCffKuCBgmv4jbe3oWygD_q_yL6ebqvBJ_Ht6QV84MGd8-v8fk--f19epLdfFtc7b6eFFZUddT1SqrXN82AICACM1227iG11YoJ61SfYdOgRNOWcncfmHeQC2lVNbJtgVxTD4svrcp_pwxT3r02ZYdTMA4Z80VF4J3UomCsgW1Keac0Onb5EeT7jUDvb-AXi6gywX0PlPNSs_7Z_t5O2L_r-Nv5AXgC5CLFHaY9E2cUwkq_8f1ESyJkqc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2623329563</pqid></control><display><type>article</type><title>Total Ginsenoside Extract from Panax ginseng Enhances Neural Stem Cell Proliferation and Neuronal Differentiation by Inactivating GSK-3β</title><source>Springer Nature</source><creator>Lin, Kai-li ; Zhang, Ji ; Chung, Hau-lam ; Wu, Xin-yi ; Liu, Bin ; Zhao, Bo-xin ; Sze, Stephen Cho-wing ; Zhou, Ping-zheng ; Yung, Ken Kin-lam ; Zhang, Shi-qing</creator><creatorcontrib>Lin, Kai-li ; Zhang, Ji ; Chung, Hau-lam ; Wu, Xin-yi ; Liu, Bin ; Zhao, Bo-xin ; Sze, Stephen Cho-wing ; Zhou, Ping-zheng ; Yung, Ken Kin-lam ; Zhang, Shi-qing</creatorcontrib><description><![CDATA[Objective
To study the effects of total ginsenosides (TG) extract from
Panax ginseng
on neural stem cell (NSC) proliferation and differentiation and their underlying mechanisms.
Methods
The migration of NSCs after treatment with various concentrations of TG extract (50, 100, or 200 µ g/mL) were monitored. The proliferation of NSCs was examined by a combination of cell counting kit-8 and neurosphere assays. NSC differentiation mediated by TG extract was evaluated by Western blotting and immunofluorescence staining to monitor the expression of nestin and microtubule associated protein 2 (MAP2). The GSK-3β/β-catenin pathway in TG-treated NSCs was examined by Western blot assay. The NSCs with constitutively active GSK-3β mutant were made by adenovirus-mediated gene transfection, then the proliferation and differentiation of NSCs mediated by TG were further verified.
Results
TG treatment significantly enhanced NSC migration (
P
<0.01 or
P
<0.05) and increased the proliferation of NSCs (
P
<0.01 or
P
<0.05). TG mediation also significantly upregulated MAP2 expression but downregulated nestin expression (
P
<0.01 or
P
<0.05). TG extract also significantly induced GSK-3β phosphorylation at Ser9, leading to GSK-3β inactivation and, consequently, the activation of the GSK-3β/β-catenin pathway (
P
<0.01 or
P
<0.05). In addition, constitutive activation of GSK-3β in NSCs by the transfection of GSK-3β S9A mutant was found to significantly suppress TG-mediated NSC proliferation and differentiation (
P
<0.01 or
P
<0.05).
Conclusion
TG promoted NSC proliferation and neuronal differentiation by inactivating GSK-3β.]]></description><identifier>ISSN: 1672-0415</identifier><identifier>EISSN: 1993-0402</identifier><identifier>DOI: 10.1007/s11655-021-3508-1</identifier><identifier>PMID: 35084698</identifier><language>eng</language><publisher>Singapore: Springer Singapore</publisher><subject>Animals ; beta Catenin - metabolism ; Cell Differentiation ; Cell Proliferation ; Ginsenosides - pharmacology ; Glycogen Synthase Kinase 3 beta - metabolism ; Medicine ; Medicine & Public Health ; Neural Stem Cells - metabolism ; Original Article ; Panax ; Plant Extracts - pharmacology ; Rats</subject><ispartof>Chinese journal of integrative medicine, 2022-03, Vol.28 (3), p.229-235</ispartof><rights>The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature 2022</rights><rights>2022. The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-86c6fd87000e0ee07bb7f724c36f5c66d9ef60f3f6c51f576027045556cf58803</citedby><cites>FETCH-LOGICAL-c344t-86c6fd87000e0ee07bb7f724c36f5c66d9ef60f3f6c51f576027045556cf58803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35084698$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Kai-li</creatorcontrib><creatorcontrib>Zhang, Ji</creatorcontrib><creatorcontrib>Chung, Hau-lam</creatorcontrib><creatorcontrib>Wu, Xin-yi</creatorcontrib><creatorcontrib>Liu, Bin</creatorcontrib><creatorcontrib>Zhao, Bo-xin</creatorcontrib><creatorcontrib>Sze, Stephen Cho-wing</creatorcontrib><creatorcontrib>Zhou, Ping-zheng</creatorcontrib><creatorcontrib>Yung, Ken Kin-lam</creatorcontrib><creatorcontrib>Zhang, Shi-qing</creatorcontrib><title>Total Ginsenoside Extract from Panax ginseng Enhances Neural Stem Cell Proliferation and Neuronal Differentiation by Inactivating GSK-3β</title><title>Chinese journal of integrative medicine</title><addtitle>Chin. J. Integr. Med</addtitle><addtitle>Chin J Integr Med</addtitle><description><![CDATA[Objective
To study the effects of total ginsenosides (TG) extract from
Panax ginseng
on neural stem cell (NSC) proliferation and differentiation and their underlying mechanisms.
Methods
The migration of NSCs after treatment with various concentrations of TG extract (50, 100, or 200 µ g/mL) were monitored. The proliferation of NSCs was examined by a combination of cell counting kit-8 and neurosphere assays. NSC differentiation mediated by TG extract was evaluated by Western blotting and immunofluorescence staining to monitor the expression of nestin and microtubule associated protein 2 (MAP2). The GSK-3β/β-catenin pathway in TG-treated NSCs was examined by Western blot assay. The NSCs with constitutively active GSK-3β mutant were made by adenovirus-mediated gene transfection, then the proliferation and differentiation of NSCs mediated by TG were further verified.
Results
TG treatment significantly enhanced NSC migration (
P
<0.01 or
P
<0.05) and increased the proliferation of NSCs (
P
<0.01 or
P
<0.05). TG mediation also significantly upregulated MAP2 expression but downregulated nestin expression (
P
<0.01 or
P
<0.05). TG extract also significantly induced GSK-3β phosphorylation at Ser9, leading to GSK-3β inactivation and, consequently, the activation of the GSK-3β/β-catenin pathway (
P
<0.01 or
P
<0.05). In addition, constitutive activation of GSK-3β in NSCs by the transfection of GSK-3β S9A mutant was found to significantly suppress TG-mediated NSC proliferation and differentiation (
P
<0.01 or
P
<0.05).
Conclusion
TG promoted NSC proliferation and neuronal differentiation by inactivating GSK-3β.]]></description><subject>Animals</subject><subject>beta Catenin - metabolism</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Ginsenosides - pharmacology</subject><subject>Glycogen Synthase Kinase 3 beta - metabolism</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Neural Stem Cells - metabolism</subject><subject>Original Article</subject><subject>Panax</subject><subject>Plant Extracts - pharmacology</subject><subject>Rats</subject><issn>1672-0415</issn><issn>1993-0402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kc9u1DAQxi0Eon_gAbggH7mkHduxkxzRsixVK6jUcra8znhxldjFTlD7CLxOH4RnqrcpHDl5rO8332i-IeQdgxMG0JxmxpSUFXBWCQltxV6QQ9Z1ooIa-MtSq4aXmskDcpTzDYBsFMjX5GBP16prD8nv6ziZgW58yBhi9j3S9d2UjJ2oS3GklyaYO7p7knd0HX6YYDHTrzin0nY14UhXOAz0MsXBO0xm8jFQE_onJIYCffKuCBgmv4jbe3oWygD_q_yL6ebqvBJ_Ht6QV84MGd8-v8fk--f19epLdfFtc7b6eFFZUddT1SqrXN82AICACM1227iG11YoJ61SfYdOgRNOWcncfmHeQC2lVNbJtgVxTD4svrcp_pwxT3r02ZYdTMA4Z80VF4J3UomCsgW1Keac0Onb5EeT7jUDvb-AXi6gywX0PlPNSs_7Z_t5O2L_r-Nv5AXgC5CLFHaY9E2cUwkq_8f1ESyJkqc</recordid><startdate>20220301</startdate><enddate>20220301</enddate><creator>Lin, Kai-li</creator><creator>Zhang, Ji</creator><creator>Chung, Hau-lam</creator><creator>Wu, Xin-yi</creator><creator>Liu, Bin</creator><creator>Zhao, Bo-xin</creator><creator>Sze, Stephen Cho-wing</creator><creator>Zhou, Ping-zheng</creator><creator>Yung, Ken Kin-lam</creator><creator>Zhang, Shi-qing</creator><general>Springer Singapore</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20220301</creationdate><title>Total Ginsenoside Extract from Panax ginseng Enhances Neural Stem Cell Proliferation and Neuronal Differentiation by Inactivating GSK-3β</title><author>Lin, Kai-li ; Zhang, Ji ; Chung, Hau-lam ; Wu, Xin-yi ; Liu, Bin ; Zhao, Bo-xin ; Sze, Stephen Cho-wing ; Zhou, Ping-zheng ; Yung, Ken Kin-lam ; Zhang, Shi-qing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-86c6fd87000e0ee07bb7f724c36f5c66d9ef60f3f6c51f576027045556cf58803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>beta Catenin - metabolism</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Ginsenosides - pharmacology</topic><topic>Glycogen Synthase Kinase 3 beta - metabolism</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Neural Stem Cells - metabolism</topic><topic>Original Article</topic><topic>Panax</topic><topic>Plant Extracts - pharmacology</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Kai-li</creatorcontrib><creatorcontrib>Zhang, Ji</creatorcontrib><creatorcontrib>Chung, Hau-lam</creatorcontrib><creatorcontrib>Wu, Xin-yi</creatorcontrib><creatorcontrib>Liu, Bin</creatorcontrib><creatorcontrib>Zhao, Bo-xin</creatorcontrib><creatorcontrib>Sze, Stephen Cho-wing</creatorcontrib><creatorcontrib>Zhou, Ping-zheng</creatorcontrib><creatorcontrib>Yung, Ken Kin-lam</creatorcontrib><creatorcontrib>Zhang, Shi-qing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chinese journal of integrative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Kai-li</au><au>Zhang, Ji</au><au>Chung, Hau-lam</au><au>Wu, Xin-yi</au><au>Liu, Bin</au><au>Zhao, Bo-xin</au><au>Sze, Stephen Cho-wing</au><au>Zhou, Ping-zheng</au><au>Yung, Ken Kin-lam</au><au>Zhang, Shi-qing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Total Ginsenoside Extract from Panax ginseng Enhances Neural Stem Cell Proliferation and Neuronal Differentiation by Inactivating GSK-3β</atitle><jtitle>Chinese journal of integrative medicine</jtitle><stitle>Chin. J. Integr. Med</stitle><addtitle>Chin J Integr Med</addtitle><date>2022-03-01</date><risdate>2022</risdate><volume>28</volume><issue>3</issue><spage>229</spage><epage>235</epage><pages>229-235</pages><issn>1672-0415</issn><eissn>1993-0402</eissn><abstract><![CDATA[Objective
To study the effects of total ginsenosides (TG) extract from
Panax ginseng
on neural stem cell (NSC) proliferation and differentiation and their underlying mechanisms.
Methods
The migration of NSCs after treatment with various concentrations of TG extract (50, 100, or 200 µ g/mL) were monitored. The proliferation of NSCs was examined by a combination of cell counting kit-8 and neurosphere assays. NSC differentiation mediated by TG extract was evaluated by Western blotting and immunofluorescence staining to monitor the expression of nestin and microtubule associated protein 2 (MAP2). The GSK-3β/β-catenin pathway in TG-treated NSCs was examined by Western blot assay. The NSCs with constitutively active GSK-3β mutant were made by adenovirus-mediated gene transfection, then the proliferation and differentiation of NSCs mediated by TG were further verified.
Results
TG treatment significantly enhanced NSC migration (
P
<0.01 or
P
<0.05) and increased the proliferation of NSCs (
P
<0.01 or
P
<0.05). TG mediation also significantly upregulated MAP2 expression but downregulated nestin expression (
P
<0.01 or
P
<0.05). TG extract also significantly induced GSK-3β phosphorylation at Ser9, leading to GSK-3β inactivation and, consequently, the activation of the GSK-3β/β-catenin pathway (
P
<0.01 or
P
<0.05). In addition, constitutive activation of GSK-3β in NSCs by the transfection of GSK-3β S9A mutant was found to significantly suppress TG-mediated NSC proliferation and differentiation (
P
<0.01 or
P
<0.05).
Conclusion
TG promoted NSC proliferation and neuronal differentiation by inactivating GSK-3β.]]></abstract><cop>Singapore</cop><pub>Springer Singapore</pub><pmid>35084698</pmid><doi>10.1007/s11655-021-3508-1</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1672-0415 |
| ispartof | Chinese journal of integrative medicine, 2022-03, Vol.28 (3), p.229-235 |
| issn | 1672-0415 1993-0402 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2623329563 |
| source | Springer Nature |
| subjects | Animals beta Catenin - metabolism Cell Differentiation Cell Proliferation Ginsenosides - pharmacology Glycogen Synthase Kinase 3 beta - metabolism Medicine Medicine & Public Health Neural Stem Cells - metabolism Original Article Panax Plant Extracts - pharmacology Rats |
| title | Total Ginsenoside Extract from Panax ginseng Enhances Neural Stem Cell Proliferation and Neuronal Differentiation by Inactivating GSK-3β |
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