Protective effect of surface‐layer proteins from four Lactobacillus strains on tumor necrosis factor‐α‐induced intestinal barrier dysfunction

BACKGROUND The intestinal epithelium is considered the first defense protection against exogenous harmful substances, playing an indispensable role in regulating intestinal health. The protection offered by surface‐layer proteins (Slps) from different Lactobacillus strains on an impaired intestinal...

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Published in:Journal of the science of food and agriculture 2022-08, Vol.102 (11), p.4446-4453
Main Authors: Zhang, Jiaojiao, Li, Qinpei, Wu, Liying, Xu, Shichen, Lu, Rongrong
Format: Article
Language:English
Subjects:
Caco‐2 cell monolayers
Electrical resistivity
Epithelium
Interleukins
intestinal barrier dysfunction
Intestine
Lactobacillus
Permeability
Proteins
Signal transduction
surface‐layer proteins
tight junction
Tumor necrosis factor
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Summary:BACKGROUND The intestinal epithelium is considered the first defense protection against exogenous harmful substances, playing an indispensable role in regulating intestinal health. The protection offered by surface‐layer proteins (Slps) from different Lactobacillus strains on an impaired intestinal barrier was investigated in this study. RESULTS Four Slps pre‐incubated for 6 h significantly prevented the reduced transepithelial electrical resistance value and increased paracellular permeability in tumor necrosis factor (TNF)‐α‐induced Caco‐2 monolayers. TNF‐α induced lower protein expression of occludin and zonula occludens‐1, and abnormal distributions of occludin and zonula occludens‐1 were ameliorated by four Slps as well. Additionally, four Slps weakened TNF‐α‐evoked interleukin‐8 secretion and nuclear factor‐κB activation. CONCLUSION Four Slps from different strains prevent the intestinal barrier from TNF‐α‐induced dysfunction through blocking the nuclear factor‐κB signaling pathway. © 2022 Society of Chemical Industry.
ISSN:0022-5142
1097-0010
DOI:10.1002/jsfa.11798