Arcopilus eremanthusum sp. nov. as sources of antibacterial and antioxidant metabolites

• Published in: Archives of microbiology 2022-02, Vol.204 (2), p.156-156, Article 156
• Main Authors: Tavares, Dérica Gonçalves, Guimarães, Sarah da Silva Costa, Piccoli, Roberta Hilsdorf, Duarte, Whasley Ferreira, Cardoso, Patrícia Gomes
• Format: Article
• Language: English
• Subjects: Acetic acid; Acids; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antimicrobial agents; Antioxidants; Bactericidal activity; Biochemistry; Biomedical and Life Sciences; Biotechnology; Caffeic acid; Carotene; Catechin; Cell Biology; Chlorogenic acid; Cinnamic acid; Coumaric acid; DNA-directed RNA polymerase; Ecology; Endophytes; Ethyl acetate; Ferulic acid; Free radicals; Fungi; Gallic acid; High performance liquid chromatography; Life Sciences; Linoleic acid; Liquid chromatography; Metabolites; Microbial Ecology; Microbiology; New species; Original Paper; p-Coumaric acid; Phenols; RNA polymerase; RNA polymerase II; Salmonella; Scavenging; Staphylococcus aureus; Sulfonic acid; Vanillin; β-Carotene
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-022-02764-6

In this study, we evaluated the antibacterial and antioxidant activities of ethyl acetate (EtOAc) extracts of Arcopilus eremanthusum sp. nov. (CML3766) isolated from E. erythopappus . The fungi were identified using the sequences of the internal transcribed spacer (ITS), large subunit (LSU), and RNA polymerase II second largest subunit ( RPB2 ). Antibacterial activity was determined using the minimum bactericidal concentration (MBC) method. Antioxidant activity was evaluated using free radical scavenging methods with 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS •+ ) assays and the β -carotene-linoleic acid system. The total phenolic compound content was measured using the Folin–Ciocalteu method. The endophytic fungal extract presented bactericidal activity, with an MBC of 2.44 and 19.5 μg/mL against Staphylococcus aureus GL 8702 and GL 5674, respectively, and 625 μg/mL for Salmonella enterica serovar Enteritidis. In addition, this fungus demonstrated an antioxidant activity of 52.30% protection in the β -carotene method. The total concentration of phenolic compounds was 23.73 mg gallic acid equivalent (GAE)/g. Ferulic acid, trans-cinnamic acid, chlorogenic acid, catechin, vanillin, p-coumaric acid, and caffeic acid were quantified using high-performance liquid chromatography with diode array detection. The results demonstrate the potential of A. eremanthusum sp. nov. to serve as a source of antibacterial and antioxidant metabolites with possible future biotechnological applications.