An additive manufacturing‐based 3D printed poly ɛ‐caprolactone/alginate sulfate/extracellular matrix construct for nasal cartilage regeneration

Various composite scaffolds with different fabrication techniques have been applied in cartilage tissue engineering. In this study, poly ɛ‐caprolactone (PCL) was printed by fused deposition modeling method, and the prepared scaffold was filled with Alginate (Alg): Alginate‐Sulfate (Alg‐Sul) hydrogel...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2022-06, Vol.110 (6), p.1199-1209
Main Authors: Zare, Pariya, Pezeshki‐Modaress, Mohamad, Davachi, Seyed Mohammad, Chahsetareh, Hadi, Simorgh, Sara, Asgari, Negin, Haramshahi, Mohammad Amin, Alizadeh, Rafieh, Bagher, Zohreh, Farhadi, Mohamad
Format: Article
Language:English
Subjects:
3D printing
alginate sulfate hydrogel
Alginates
Alginates - chemistry
Alginates - pharmacology
Alginic acid
Biomimetics
Caproates
Cartilage
cartilage tissue engineering
Cell viability
Chondrogenesis
ECM
Extracellular matrix
Extracellular Matrix - chemistry
Fabrication
Fused deposition modeling
Glycosaminoglycans
Hydrogels
Lactones
Mechanical properties
Mesenchyme
Nasal Cartilages
polycaprolactone
Printing, Three-Dimensional
Regeneration
Scaffolds
Stem cells
Sulfates
Three dimensional printing
Tissue engineering
Tissue Engineering - methods
Tissue Scaffolds - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Various composite scaffolds with different fabrication techniques have been applied in cartilage tissue engineering. In this study, poly ɛ‐caprolactone (PCL) was printed by fused deposition modeling method, and the prepared scaffold was filled with Alginate (Alg): Alginate‐Sulfate (Alg‐Sul) hydrogel to provide a better biomimetic environment and emulate the structure of glycosaminoglycans properly. Furthermore, to enhance chondrogenesis, different concentrations of decellularized extracellular matrix (dECM) were added to the hydrogel. For cellular analyses, the adipose‐derived mesenchymal stem cells were seeded on the hydrogel and the results of MTT assay, live/dead staining, and SEM images revealed that the scaffold with 1% dECM had better viscosity, cell viability, and proliferation. The study was conducted on the optimized scaffold (1% dECM) to determine mechanical characteristics, chondrogenic differentiation, and results demonstrated that the scaffold showed mechanical similarity to the native nasal cartilage tissue along with possessing appropriate biochemical features, which makes this new formulation based on PCL/dECM/Alg:Alg‐Sul a promising candidate for further in‐vivo studies.
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.37363