Chia expeller: A promising source of antioxidant, antihypertensive and antithrombotic peptides produced by enzymatic hydrolysis with Alcalase and Flavourzyme

• Published in: Food chemistry 2022-06, Vol.380, p.132185-132185, Article 132185
• Main Authors: Ozón, Brenda, Cotabarren, Juliana, Valicenti, Tania, Graciela Parisi, Mónica, David Obregón, Walter
• Format: Article
• Language: English
• Subjects: Antihypertensive Agents; Antihypertensive peptides; Antioxidant compounds; Antioxidants; Antithrombotic peptides; Chia by-products; Endopeptidases; Enzymatic hydrolysates; Expeller; Fibrinolytic Agents; Hydrolysis; Peptides; Protein Hydrolysates; Salvia hispanica L; Sequential hydrolysis; Subtilisins
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2022.132185

•Chia expeller is a promising source of potential BAPs produced by enzymatic hydrolysis.•A higher degree of hydrolysis was obtained by 90 min-Alcalase and 90 min-Flavourzyme (H-A90-F90).•H-A90-F90 showed antiradical activity slightly higher than those observed for other native crops.•H-A90-F90 showed antithrombotic activity against intrinsic and extrinsic coagulation pathways.•H-A90-F90 showed ACE inhibitory activity presenting potential as antihypertensive peptides. Chia expeller is a promising source of bioactive compounds suitable for the development of nutraceutical ingredients due to its functional, biological, and nutritional properties. In this work, chia expeller was hydrolysed with Alcalase-Flavourzyme sequential system and compared to the individual enzymes. A higher degree of hydrolysis (57.63 ± 6.08%) was obtained after 90 min-Alcalase and 90 min-Flavourzyme (H-A90-F90), with the development of low molecular weight peptides as observed by SDS-PAGE. H-A90-F90 exhibited antiradical activity with ABTS (TE = 4.87 ± 0.13 mmol L-1 mg−1), DPPH (TE = 1.55 ± 0.02 mmol L-1 mg−1), antihypertensive activity (45% ACE-I inhibition), and antithrombotic activity against both intrinsic and extrinsic coagulation pathways. These results represent the first report of antithrombotic peptides from Salvia hispanica, highlighting the relevant use of chia seed by-products to obtain potentially antioxidant, antihypertensive, and anticoagulant peptides by enzymatic hydrolysis with Alcalase and Flavourzyme, enhancing this agro-industrial by-product.