Novel insights from lignocellulosic waste to biogas through regulated dry-wet combined anaerobic digestion: Focusing on mining key microbes

[Display omitted] •Established a high lignocellulosic dry-wet combined anaerobic digestion system.•The kinetic parameters of the whole anaerobic digestion process were revealed.•Synergism between Clostridiales and methanogens was evaluated via network analysis.•Syntrophy between SFOB and methylotrop...

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Published in:Bioresource technology 2022-03, Vol.348, p.126778-126778, Article 126778
Main Authors: Liang, Yi, Zhao, Lixin, Zhao, Yubin, Li, Zaixing, Feng, Jing, Yao, Zonglu, Ye, Bingnan, Chen, Jiankun, Ning, Zhifang, Li, Peiqi, Yu, Jiadong
Format: Article
Language:English
Subjects:
Anaerobiosis
Biofuels
Bioreactors
Dry-wet combined anaerobic digestion
High solid content
Lignin
Lignocellulosic waste
Methane
Microbial community
Recombination regulation
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Summary:[Display omitted] •Established a high lignocellulosic dry-wet combined anaerobic digestion system.•The kinetic parameters of the whole anaerobic digestion process were revealed.•Synergism between Clostridiales and methanogens was evaluated via network analysis.•Syntrophy between SFOB and methylotrophic Methanospirillum was observed.•The number of key acidogenic Clostridium was increased by 80.77–107.11%. Dry-wet combined anaerobic digestion is a novel approach for treating lignocellulosic waste by increasing the organic load of reactor while accelerating the conversion of organic acids. Here, we investigated the effect of regulated substrate ratios and initial pH in the dry acidogenesis stage on the bioconversion efficiency of dry-wet combined anaerobic digestion. Our data revealed microbial interactions and further identified key microbes based on microbial co-occurrence network analysis. On day three of acidification, the kinetic hydrolysis rate and acidification yield reached 1.66 and 60.07%, respectively; this was attributed to enhancement of the synergistic effect between Clostridiales and Methanosaeta, which increased the proportion of corn straw in the substrate or lowered the initial spray slurry pH to 5.5–6.5. With increased acidification capacity, acetoclastic methanogens were enriched in the wet methanogenesis stage; the syntrophic effect of Syntrophomonadales, Syntrophobacterales and Methanospirillum, meanwhile, was enhanced, leading to an overall improvement in biogas production.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2022.126778