Impact of selenium on rhizosphere microbiome of a hyperaccumulation plant Cardamine violifolia

• Published in: Environmental science and pollution research international 2022-06, Vol.29 (26), p.40241-40251
• Main Authors: Guo, Zisheng, Zhu, Bin, Guo, Jia, Wang, Gongting, Li, Meng, Yang, Qiaoli, Wang, Liping, Fei, Yue, Wang, Shiwei, Yu, Tian, Sun, Yanmei
• Format: Article
• Language: English
• Subjects: Abundance; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Cardamine; Earth and Environmental Science; Ecotoxicology; Enrichment; Environment; Environmental Chemistry; Environmental Health; Environmental science; Fertilizers; Microbiomes; Microbiota; Microorganisms; Nanoparticles; Nutrient content; Research Article; Rhizosphere; Saccharomyces cerevisiae; Selenic Acid; Selenious Acid; Selenite; Selenites; Selenium; Selenium - analysis; Selenium Compounds; Soil; Soils; Waste Water Technology; Water Management; Water Pollution Control; Yeast; Yeasts
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-022-18974-w

Cardamine violifolia is the only selenium hyperaccumulation plant found in China. It has been developed as a source of medicinal and edible products that we can consume as selenium supplements. Many planting approaches have been developed to increase the selenium content of C. violifolia for nutrient biofortification. However, the contribution of rhizosphere microbes of C. violifolia to selenium enrichment has not been investigated. In this study, four types of selenium, i.e., selenate, selenite, nanoparticles selenium from Bacillus subtilis ( B. subtilis -Se), and organic selenium from yeast (yeast-Se), were added to the soil that C. violifolia was grown in, respectively. Selenate led to the greatest accumulation of selenium in C. violifolia , followed by selenite, B. subtilis -Se, and yeast-Se. Except for yeast-Se, the concentration of selenium in C. violifolia positively correlated with the amount of selenium added to the soil. Furthermore, the different types of exogenous selenium exhibited distinct effects on the rhizosphere microbiome of C. violifolia . Alpha and beta diversity analyses demonstrated that rhizosphere microbiome was more obviously affected by selenium from B. subtilis and yeast than from selenate and selenite. Different microbial species were enriched in the rhizosphere of C. violifolia under various exogenous selenium treatments. B. subtilis -Se application enhanced the abundance of Leucobacter , Sporosarcina , Patulibacter , and Denitrobacter , and yeast-Se application enriched the abundance of Singulishaera , Lactobacillus , Bdellovibrio , and Bosea . Bosea and the taxon belonging to the order Solirubrobacterales were enriched in the samples with selenate and selenite addition, respectively, and the abundances of these were linearly related to the concentrations of selenate and selenite applied in the rhizosphere of C. violifolia . In summary, this study revealed the response of the rhizosphere microbiome of C. violifolia to exogenous selenium. Our findings are useful for developing suitable selenium fertilizers to increase the selenium hyperaccumulation level of this plant.