Enhanced production of Bacopa saponins by repeated batch strategy in bioreactor

• Published in: Bioprocess and biosystems engineering 2022-05, Vol.45 (5), p.829-841
• Main Authors: Sahoo, Krishna Kalyani, Sahu, Binod B., Singh, V. R., Patra, Nivedita
• Format: Article
• Language: English
• Subjects: Bioreactors; Biotechnology; Cell culture; Chemistry; Chemistry and Materials Science; Cultivation; Engineering; Environmental Engineering/Biotechnology; Food Science; Immune system; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Mass transfer; Physiology; Research Paper; Saponins; Suspension culture
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-022-02700-4

Cultivation of cell suspension culture of Bacopa monnieri targeting the production of bacosides was explored in a 5-l stirred tank reactor using statistically optimized conditions. The bioreactor cultivation conditions were modified and this led to profuse biomass growth (2.81 ± 0.20 g/l) and total bacosides (1.26 ± 0.23 mg/g in cells and 0.60 ± 0.11 mg/l in fermenter broth) production in 9 days. The values of static volumetric mass transfer coefficient ( k L a), dimensionless mixing time ( N m ) were measured in the bioreactor. The culture grew efficiently and produced enhanced amount of bacoside A (5.59 ± 0.41 mg/g total bacosides in cells and 3.12 ± 0.13 mg/l in the fermenter broth) using one cycle of repeated batch strategy adopted in the bioreactor for 15 days. The intracellular concentration of bacoside A3 (1.18 ± 0.11 mg/g), bacopaside II (2.09 ± 0.35 mg/g), bacopaside X (0.79 ± 0.17 mg/g) and bacopasaponin C (2.24 ± 0.23 mg/g) were significantly higher in repeated batch as compared to batch bioreactor cultivation. The yield of total bacosides in the fermenter broth was 5-times higher in repeated batch as compared to batch cultivation. This strategy can be helpful for the enhanced production of other valuable triterpenoid saponins.