An in situ hybridization study of syndecan family during the late stages of developing mouse molar tooth germ

Expression of syndecan - 1, 2, 3 , and 4 mRNAs during the late stages of tooth germ formation was investigated by in situ hybridization, using [ 35 S]-UTP-labeled cRNA probes. Syndecan - 1 mRNA was mainly expressed in the stellate reticulum and stratum intermedium as well as at the cervical region o...

Full description

Saved in:
Bibliographic Details
Published in:Anatomical science international 2022-09, Vol.97 (4), p.358-368
Main Authors: Fujikawa, Kaoru, Nonaka, Naoko, Wang, Xiaofang, Shibata, Shunichi
Format: Article
Language:English
Subjects:
Anatomy
Animal Anatomy
Animal Physiology
Cell Biology
Collagen (type I)
Dentinogenesis
Epithelium
Gene expression
Histology
Human Physiology
Hybridization
Medicine
Medicine & Public Health
Morphology
Neurosciences
Odontoblasts
Original Article
Polymerase chain reaction
Syndecan
Teeth
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Expression of syndecan - 1, 2, 3 , and 4 mRNAs during the late stages of tooth germ formation was investigated by in situ hybridization, using [ 35 S]-UTP-labeled cRNA probes. Syndecan - 1 mRNA was mainly expressed in the stellate reticulum and stratum intermedium as well as at the cervical region of dental papilla/dental follicle during E18.5-P3.0. Expression in the dental epithelium was enhanced during the postnatal periods, which was supported by real-time RT-PCR analysis. These spatiotemporal expression patterns may suggest specific roles of syndecan-1 in tooth formation such as tooth eruption or root formation. Syndecan - 3 mRNA expression became evident in odontoblasts at E18.5, but compared to collagen type I mRNA, which was strongly expressed at this stage, syndecan-3 expression in odontoblast was restricted in mature odontoblasts beneath the cusps during the postnatal periods. This result was also supported by real-time RT-PCR analysis, and indicated that syndecan-3 may be involved in the progress of dentinogenesis rather than in the initiation of it. Syndecan-4 mRNA roughly showed comparable expression patterns to those of syndecan-3 . Syndecan-2 mRNA did not show significant expression during the experimental period, but real-time RT-PCR analysis suggested that syndecan-2 expression might be enhanced with hard tissue formation.
ISSN:1447-6959
1447-073X
DOI:10.1007/s12565-022-00647-w