Identification of a novel laccase gene EuLAC1 and its potential resistance against Botrytis cinerea

In this study, a novel laccase gene, EuLAC1 , was cloned from Eucommia ulmoides Oliver ( E. ulmoides ). An overexpression vector harboring the EuLAC1 was constructed and introduced into the tobacco ( Nicotiana tabacum cv. Xanthi). The laccase activity, resistance to Botrytis cinerea ( B. cinerea ) a...

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Bibliographic Details
Published in:Transgenic research 2022-04, Vol.31 (2), p.215-225
Main Authors: Zhao, Yichen, Liu, Yuqian, Dong, Xuan, Liu, Jia-Jia, Zhao, De-Gang
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Botrytis - genetics
Botrytis cinerea
Catheters
Cell walls
Eucommia ulmoides
Genetic Engineering
Grey mold
Laccase
Laccase - genetics
Life Sciences
Lignin
Lignin - genetics
Molecular Medicine
Nicotiana - genetics
Nicotiana tabacum
Original Paper
Plant Diseases - genetics
Plant Genetics and Genomics
Plants, Genetically Modified - genetics
Scanning electron microscopy
Stems
Tobacco
Transgenic plants
Transgenics
Xylem
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Summary:In this study, a novel laccase gene, EuLAC1 , was cloned from Eucommia ulmoides Oliver ( E. ulmoides ). An overexpression vector harboring the EuLAC1 was constructed and introduced into the tobacco ( Nicotiana tabacum cv. Xanthi). The laccase activity, resistance to Botrytis cinerea ( B. cinerea ) and lignin level in wild-type and transgenic plants were thereafter investigated. Interestingly, the transgenic tobacco displayed a significantly higher laccase activity and resistance to gray mold as compared to the wild-type tobacco. Additionally, the lignin contents in the leaves and stems of the transgenic tobacco were significantly higher in comparison to the wild-type tobacco. Scanning electron microscopy was used to observe the cross sections of wild-type and transgenic tobacco stems and it was noted that the cell wall near the xylem catheter of the transgenic tobacco was substantially thicker and the outline clearer than that of the wild-type. Thus, the EuLAC1 gene can significantly increase laccase activity and lignin content in tobacco, leading to an increase in the physical defenses, thereby increasing tobacco resistance to gray mold.
ISSN:0962-8819
1573-9368
DOI:10.1007/s11248-022-00297-8