Hepatic transcriptomic and histopathological responses of common carp, Cyprinus carpio, to copper and microplastic exposure

• Published in: Marine pollution bulletin 2022-02, Vol.175, p.113401-113401, Article 113401
• Main Authors: Hoseini, Seyyed Morteza, Khosraviani, Kave, Hosseinpour Delavar, Fatemeh, Arghideh, Mohammad, Zavvar, Fatemeh, Hoseinifar, Seyed Hossein, Van Doan, Hien, Zabihi, Erfan, Reverter, Miriam
• Format: Article
• Language: English
• Subjects: Accumulation; Animals; Apoptosis; Bioaccumulation; Biomarkers; Carps - metabolism; Co-exposure; Copper; Copper - pharmacology; Cyprinus carpio; Detoxification; Exposure; Fish; Freshwater fishes; Gene expression; Gills - metabolism; Heavy metals; Hepatic damage; Histopathology; Lesions; Leukocytes; Liver; Liver - metabolism; Microparticles; Microplastics; Plastics - metabolism; Polyvinyl chloride; PVC microparticle; Transcription; Transcriptome; Transcriptomics; Water Pollutants, Chemical - metabolism
• ISSN: 0025-326X; 1879-3363
• DOI: 10.1016/j.marpolbul.2022.113401

The combined effects of copper and polyvinyl chloride (PVC) microparticles were investigated on the metal accumulation, histopathological biomarkers, and targeted transcriptomics in Cyprinus carpio liver. The fish were exposed to 0.25 mg/L copper and/or 0.5 mg/L PVC microparticles over a 14-d period. The results showed that hepatic copper accumulation is facilitated by the PVC microparticles presence in water. All treatments induced significant hepatic stress and inflammation; however, the transcriptional responses involving in detoxification pathways and apoptotic mechanisms were mixed and often down-regulated in the fish exposed to copper and/or PVC microparticles. Exposure to copper and/or PVC microparticles induced hypermeia, leukocyte infiltration and increase in melanomacrophage centers number and area. Generally, the severity of the lesions was in the following order: PVC microparticles