Selection of Aptamers for Sensing Caffeine and Discrimination of Its Three Single Demethylated Analogues

With the growing consumption of caffeine-containing beverages, detection of caffeine has become an important biomedical, bioanalytical, and environmental topic. We herein isolated four high-quality aptamers for caffeine with dissociation constants ranging from 2.2 to 14.6 μM as characterized using i...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2022-02, Vol.94 (7), p.3142-3149
Main Authors: Huang, Po-Jung Jimmy, Liu, Juewen
Format: Article
Language:English
Subjects:
Analytical chemistry
Aptamers
Beverages
Caffeine
Calorimetry
Chemistry
Fluorescence
Humans
Oligonucleotides
Theobromine
Theophylline
Titration
Titration calorimetry
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Summary:With the growing consumption of caffeine-containing beverages, detection of caffeine has become an important biomedical, bioanalytical, and environmental topic. We herein isolated four high-quality aptamers for caffeine with dissociation constants ranging from 2.2 to 14.6 μM as characterized using isothermal titration calorimetry. Different binding patterns were obtained for the three single demethylated analogues: theobromine, theophylline, and paraxanthine, highlighting the effect of the molecular symmetry of the arrangement of the three methyl groups in caffeine. A structure-switching fluorescent sensor was designed showing a detection limit of 1.2 μM caffeine, which reflected the labeled caffeine concentration within 6.1% difference for eight commercial beverages. In 20% human serum, a detection limit of 4.0 μM caffeine was achieved. With the four aptamer sensors forming an array, caffeine and the three analogues were well separated from nine other closely related molecules.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.1c04349