Evidence of coevolution between Cronartium harknessii lineages and their corresponding hosts, lodgepole pine and jack pine

• Published in: Phytopathology 2022-08, Vol.112 (8), p.1795-1807
• Main Authors: McAllister, Chandra, Cullingham, Catherine, Peery, Rhiannon, Mbenoun, Michael, McPeak, Eden, Feau, Nicholas, Hamelin, Richard, Ramsfield, Tod, Myrholm, Colin, Cooke, Janice Elizabeth
• Format: Article
• Language: English
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/PHYTO-09-21-0370-R

Variation in susceptibility and rate of infection of spp. to the fungus (E. Meinecke (syn (J.P. Moore)) Y Hiratsuka), the causative agent of western gall rust, has been well documented. To test the hypothesis that there is a coevolutionary relationship between and its hosts, we examined genetic structure and virulence of associated with lodgepole pine ( Douglas ex Loudon var. Engelm.), jack pine ( Lamb) and their hybrids. A secondary objective was to improve assessment and diagnosis of infection in hosts. Using 18 microsatellites, we assessed genetic structure of from 90 sites within the ranges of lodgepole pine and jack pine. We identified two lineages (East and West, FST = 0.677) associated with host genetic structure (r = 0.81, p = 0.001), with East comprising three sub-lineages. In parallel, we conducted a factorial experiment in which lodgepole pine, jack pine and hybrid seedlings were inoculated with spores from the two primary genetic lineages. With this experiment we refined the phenotypic categories associated with infection, and demonstrated that stem width can be used as a quantitative measure of host response to infection. Overall, each host responded differentially to the fungal lineages, with jack pine exhibiting more resiliency to infection than lodgepole pine, and hybrids exhibiting intermediate resiliency. Taken together, the shared genetic structure between fungus and host species, and the differential interaction of the fungal species with the hosts, supports a coevolutionary relationship between host and pathogen.