The effect of Anzer honey on X‐ray induced genotoxicity in human lymphocytes: An in vitro study

Anzer honey is well known in Turkey and used for its medicinal properties, especially for pharyngitis, tonsillitis, ulcers and cancer. In this study, we investigated whether Anzer honey, which is shown to have antioxidant, anti‐tumoral, and anti‐inflammatory properties, has a protective effect again...

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Published in:Microscopy research and technique 2022-06, Vol.85 (6), p.2241-2250
Main Authors: Bagatir, Gulcin, Kaya, Murat, Suer, Ilknur, Cefle, Kıvanc, Palanduz, Ayse, Palanduz, Sukru, Becerir, Hatice Bilge, Koçyiğit, Mine, Ozturk, Sukru
Format: Article
Language:English
Subjects:
Antioxidants
Anzer honey
Blood
chromosomal breakage
Chromosome banding
Cytogenetics
Cytokinesis
Damage prevention
Genotoxicity
Honey
Inflammation
Lymphocytes
micronucleus
Oxidation
Peripheral blood
Pharyngitis
Radiation
Radiation damage
Tonsillitis
Ulcers
X‐radiation
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Summary:Anzer honey is well known in Turkey and used for its medicinal properties, especially for pharyngitis, tonsillitis, ulcers and cancer. In this study, we investigated whether Anzer honey, which is shown to have antioxidant, anti‐tumoral, and anti‐inflammatory properties, has a protective effect against X‐ray induced genotoxic damage by cytogenetic methods. Peripheral blood lymphocytes isolated from 20 healthy volunteers were divided into two groups and cultivated by conventional methods. Study group lymphocytes were treated with 10% diluted honey while those in the control group were not. Both groups were exposed to a high dose (2 Gy) X‐ray at the 48th hour of culture. Conventional cytogenetic staining and Giemsa banding methods were applied to evaluate chromosomal breakage and ring formation. Micronucleus frequencies were determined by the cytokinesis‐block micronucleus (CBMN) assay. Paired sample t test was used to compare groups. Anzer honey, which was analyzed melissopalynologically, was used. Micronucleus frequency was significantly decreased in the study group (CI = 348.75 ± 31, median 326, min. 98, max. 704) compared to the control group (CI = 489.10 ± 27, median 500, min. 216, max. 645) (p = .001). Chromosomal breakage was also significantly decreased in the study group (CI = 118.70 ± 16, median 109, min. 12, max. 316) compared to the control group (CI = 233.60 ± 25, median 225, min. 65, max. 492) (p 
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.24081