Macrolactin Metabolite Production by Bacillus sp. ZJ318 Isolated from Marine Sediment

A total of 172 microbial strains were screened and isolated from Arctic Ocean marine sediments at a depth of 42 ~ 3,763 m. A microorganism with strong antibacterial activity against Staphylococcus aureus was identified as Bacillus sp. ZJ318 according to the results of 16S rDNA sequencing and phyloge...

Full description

Saved in:
Bibliographic Details
Published in:Applied biochemistry and biotechnology 2022-06, Vol.194 (6), p.2581-2593
Main Authors: Zhang, Liying, Jin, Meishu, Shi, Xian, Jin, Liming, Hou, Xiyan, Yu, Yong, Liu, Baoquan, Cao, Jijuan, Quan, Chunshan
Format: Article
Language:English
Subjects:
Acetic acid
Ammonium sulfate
Antibacterial activity
Bacillus
Biochemistry
Biological activity
Biotechnology
Chemistry
Chemistry and Materials Science
Ethyl acetate
Fermentation
Fractionation
High performance liquid chromatography
Ionization
Liquid chromatography
Literature reviews
Marine microorganisms
Marine sediments
Metabolites
Microorganisms
Nitrogen sources
NMR
Nuclear magnetic resonance
Original Article
Phylogeny
rRNA 16S
Sediments
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A total of 172 microbial strains were screened and isolated from Arctic Ocean marine sediments at a depth of 42 ~ 3,763 m. A microorganism with strong antibacterial activity against Staphylococcus aureus was identified as Bacillus sp. ZJ318 according to the results of 16S rDNA sequencing and phylogenetic tree analyses. Bioactivity-guided isolation of the new/novel metabolite in the ethyl acetate (EA) extract obtained from the fermentation broth of this strain was followed by chromatographic fractionation and subsequent HPLC purification, leading to the isolation of one known macrolactin. The chemical structure of the macrolactin, which indicated macrolactin J isolation from marine microorganisms for the first time, was assigned based on a high-resolution electrospray ionization mass spectrometer system (HR-EMI-MS), nuclear magnetic resonance (NMR) spectral analyses, and a literature review. To improve macrolactin J production, the corresponding effects of nitrogen sources were investigated, and (NH 4 ) 2 SO 4 was determined to produce the best effect. In addition, the optimal culture conditions were determined by an orthogonal experiment. Under these conditions, the yield of macrolactin J was increased to 2.41 mg/L, which was 2.2 times the original yield. This work lays a foundation for follow-up mechanistic and application research on macrolactin J.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-022-03841-8